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https://hdl.handle.net/2440/10154
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Type: | Journal article |
Title: | Methionine derivatives diminish sulphide damage to colonocytes - implications for ulcerative colitis. |
Author: | Roediger, W. Babidge, W. Millard, S. |
Citation: | Gut, 1996; 39(1):77-81 |
Publisher: | B M J PUBLISHING GROUP |
Issue Date: | 1996 |
ISSN: | 0017-5749 1468-3288 |
Abstract: | <h4>Background</h4>Bacterial production of anionic sulphide is increased in the colon of ulcerative colitis and sulphides can cause metabolic damage to colonocytes.<h4>Aims</h4>To assess the reversal of the damaging effect of sulphide to isolated colonocytes by methionine and methionine derivatives.<h4>Methods and subjects</h4>Isolated colonocytes were prepared from rat colons and 12 human colectomy specimens. In cell suspensions 14CO2/acetoacetate generation was measured from [1-14C]-butyrate (5.0 mmol/l) in the presence of 0-2.0 mmol/l sodium hydrogen sulphide. The effect of 5.0 mmol/l L-methionine, S-adenosylmethionine 1,4 butane disulphonate and DL-methionine-S-methylsulphonium chloride on sulphide inhibited oxidation was observed.<h4>Results</h4>In rat colonocytes sodium hydrogen sulphide dose dependently reduced oxidative metabolite formation from n-butyrate, an action reversed in order of efficacy by S-adenosylmethionine 1,4 butane disulphonate > DLmethionine-S-methyl-sulphonium chloride > L-methionine. In human colonocytes S-adenosylmethionine 1,4 butane disulphonate most significantly improved 14CO2 production (p = < 0.005) suppressed by sodium hydrogen sulphide.<h4>Conclusion</h4>Sulphide toxicity in colonocytes is reversible by methyl donors. The efficiency of sulphide detoxification may be an important factor in the pathogenesis and treatment of ulcerative colitis for which S-adenosylmethionine 1,4 butane disulphonate may be of therapeutic value. |
Keywords: | Colon Cells, Cultured Animals Humans Rats Rats, Sprague-Dawley Colitis, Ulcerative Carbon Dioxide Sulfides Carbon Radioisotopes Acetamides Butyric Acid Butyrates Vitamin U Methionine S-Adenosylmethionine Scintillation Counting Male |
DOI: | 10.1136/gut.39.1.77 |
Published version: | http://dx.doi.org/10.1136/gut.39.1.77 |
Appears in Collections: | Aurora harvest 7 Surgery publications |
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