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https://hdl.handle.net/2440/11539
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Type: | Journal article |
Title: | Neutrophils activated by granulocyte-macrophage colony-stimulating factor express receptors for interleukin-3 which mediate class II expression |
Author: | Smith, W. Guida, L. Qiuy, S. Korpelainen, E. van den Hueven, C. Gillis, D. Hawrylowicz, C. Vadas, M. Lopez, A. |
Citation: | Blood, 1995; 86(10):3938-3944 |
Publisher: | American Society of Hematology |
Issue Date: | 1995 |
ISSN: | 0006-4971 1528-0020 |
Statement of Responsibility: | WB Smith, L Guida, Q Sun, EI Korpelainen, C van den Heuvel, D Gillis, CM Hawrylowicz, MA Vadas, and AF Lopez |
Abstract: | Freshly isolated peripheral blood neutrophils, unlike monocytes and eosinophils, do not bind interleukin-3 (IL-3) or respond to IL-3). We show that neutrophils cultured for 24 hours in granulocyte-macrophage colony-stimulating factor (GM-CSF) express mRNA for the IL-3 receptor (R) alpha subunit, as shown by RNase protection assays, and IL-3R alpha chain protein, as shown by cytometric analysis using two different specific monoclonal antibodies. This effect was selective for GM-CSF, because granulocyte colony-stimulating factor, tumor necrosis factor- alpha, interferon-gamma, and IL-1 failed to induce the IL-3 receptor. Saturation binding curves with 125I-IL-3 and Scatchard transformation showed the presence of about 100 high-affinity and 4,000 low-affinity receptors. Because neutrophils have been shown to express human leukocyte antigen (HLA)-DR in response to GM-CSF, we examined the possibility that IL-3 could augment HLA-DR expression on GM-CSF-treated cells. We found that neutrophils incubated with 30 ng/mL IL-3 as well as 0.1 ng/mL GM-CSF expressed a mean of 2.1-fold higher levels of HLA- DR than with GM-CSF alone (P < .005), confirming the signaling competence of the newly expressed IL-3R. This increase was seen even at maximal concentrations of GM-CSF and IL-3 can have an additive effect on mature human cells. The augmentation of HLA-DR by IL-3 was specific because it could be inhibited by a blocking anti-IL-3R antibody. Expression of class II molecules by neutrophils under these conditions may have significance for antigen presentation. These results provide further evidence for the role of GM-CSF as an amplification factor in inflammation by inducing neutrophil responsiveness to IL-3 produced by T cells or mast cells. |
Keywords: | Neutrophils Cells, Cultured Humans Granulocyte-Macrophage Colony-Stimulating Factor Interleukin-3 Receptors, Granulocyte-Macrophage Colony-Stimulating Factor Receptors, Interleukin-3 Recombinant Proteins RNA, Messenger HLA-D Antigens Flow Cytometry Signal Transduction Neutrophil Activation Gene Expression Regulation |
Rights: | © 1995 by The American Society of Hematology |
DOI: | 10.1182/blood.v86.10.3938.bloodjournal86103938 |
Published version: | http://www.bloodjournal.org/content/86/10/3938 |
Appears in Collections: | Aurora harvest 2 Microbiology and Immunology publications |
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