Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/11582
Citations | ||
Scopus | Web of Science® | Altmetric |
---|---|---|
?
|
?
|
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Paton, A. | - |
dc.contributor.author | Bourne, A. | - |
dc.contributor.author | Manning, P. | - |
dc.contributor.author | Paton, J. | - |
dc.date.issued | 1995 | - |
dc.identifier.citation | Infection and Immunity, 1995; 63(7):2450-2458 | - |
dc.identifier.issn | 0019-9567 | - |
dc.identifier.issn | 1098-5522 | - |
dc.identifier.uri | http://hdl.handle.net/2440/11582 | - |
dc.description.abstract | Shiga-like toxin (SLT)-producing strains of Escherichia coli are known to cause diarrhea, hemorrhagic colitis, and hemolytic-uremic syndrome in humans. The SLTs, particularly those related to type II (SLT-II), are a diverse family of toxins which may have differing in vitro or in vivo properties. To examine the impact of naturally occurring SLT-II sequence variation on the capacity of a given E. coli strain to cause disease, operons encoding four different SLT-II-related toxins, designated SLT-II/O111, SLT-II/OX3a, SLT-II/OX3b, and SLTII/ O48, were cloned in the same orientation in pBluescript. French pressure cell lysates of E. coli DH5a derivatives carrying these plasmids differed markedly in cytotoxicity for Vero cells, with 50% cytotoxic doses ranging from 20 to 328,000/ml. The strains also differed in oral virulence for streptomycin-treated mice, as judged by survival rate and/or median survival time, but virulence did not necessarily correlate with in vitro cytotoxicity. The SLT-II type associated with the lowest oral virulence was SLT-II/O111. Both the overall survival rate and the median survival time of mice challenged with clones producing this toxin were significantly greater than that for mice challenged with a clone producing the closely related SLT-II/OX3a. Experiments with clones carrying chimeric O111/OX3a SLT-II operons indicated that the reduced virulence was associated with an Arg-1763Gly substitution in the mature A subunit. Clones producing SLT-II/O48 and SLT-II/OX3b had similarly high cytotoxicities for Vero cells, but the latter was more virulent when fed to streptomycin-treated mice, as judged by median survival time. Experiments with clones carrying chimeric O48/OX3b SLT-II operons indicated that the increased virulence was a function of the A subunit of SLT-II/ OX3b, which differs from the A subunit of SLT-II/O48 by only two amino acids (Met-43Thr and Gly-1023Asp, respectively). These findings raise the possibility that naturally occurring SLT-II sequence variations may impact directly on the capacity of a given SLT-producing E. coli strain to cause disease. | - |
dc.language.iso | en | - |
dc.publisher | American Society for Microbiology | - |
dc.rights | © 1995, American Society for Microbiology | - |
dc.source.uri | http://iai.asm.org/content/63/7/2450 | - |
dc.subject | Kidney | - |
dc.subject | Hela Cells | - |
dc.subject | Vero Cells | - |
dc.subject | Animals | - |
dc.subject | Mice, Inbred BALB C | - |
dc.subject | Humans | - |
dc.subject | Mice | - |
dc.subject | Escherichia coli | - |
dc.subject | Recombinant Fusion Proteins | - |
dc.subject | Oligonucleotide Probes | - |
dc.subject | Bacterial Toxins | - |
dc.subject | Enterotoxins | - |
dc.subject | Mutagenesis, Site-Directed | - |
dc.subject | Sequence Alignment | - |
dc.subject | Amino Acid Sequence | - |
dc.subject | Base Sequence | - |
dc.subject | Sequence Homology, Amino Acid | - |
dc.subject | Structure-Activity Relationship | - |
dc.subject | Genes, Bacterial | - |
dc.subject | Operon | - |
dc.subject | Molecular Sequence Data | - |
dc.subject | Male | - |
dc.subject | Shiga Toxin 2 | - |
dc.subject | Chlorocebus aethiops | - |
dc.title | Comparative toxicity and virulence of Escherichia coli clones expressing variant and chimeric Shiga-like toxin type II operons | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1128/iai.63.7.2450-2458.1995 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Paton, J. [0000-0001-9807-5278] | - |
Appears in Collections: | Aurora harvest 7 Microbiology and Immunology publications |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.