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https://hdl.handle.net/2440/11619
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DC Field | Value | Language |
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dc.contributor.author | Qiao, M. | - |
dc.contributor.author | Scougall, C. | - |
dc.contributor.author | Duszynski, A. | - |
dc.contributor.author | Burrell, C. | - |
dc.date.issued | 1999 | - |
dc.identifier.citation | Journal of General Virology, 1999; 80(8):2127-2135 | - |
dc.identifier.issn | 0022-1317 | - |
dc.identifier.issn | 1465-2099 | - |
dc.identifier.uri | http://hdl.handle.net/2440/11619 | - |
dc.description | © 1999 Society for General Microbiology | - |
dc.description.abstract | This paper describes the use of one-step growth conditions to study the kinetics of duck hepatitis B virus (DHBV) replication in primary duck hepatocytes. Synchronized infection was achieved using partially purified DHBV virions at an m.o.i. of 640 DHBV DNA-containing virions per cell, and these conditions were shown to produce a single cycle of infection. In this model, input purified DHBV DNA was rapidly internalized by cells at 0·5 h, and localized to the nucleus by 4 h, but both covalently closed circular (CCC) DNA and single-stranded DNA were not detected until 48 h post-inoculation (p.i.), suggesting that there was a 40 h delay between DHBV localization to the nucleus and formation of CCC DNA. In contrast, CCC DNA can be first detected in hepatocytes at 6 h p.i. in in vivo infection of ducks with the same DHBV strain. In an analysis of the nuclear transport of the DHBV genome, release of nuclear viral DNA from a particulate form to a soluble nucleoplasmic form was only 50% complete by 48 h p.i. However, this process occurred simultaneously with genome uncoating since all soluble nucleoplasmic DHBV DNA was free of nucleocapsid material; this suggests that nucleocapsid disassembly and genome uncoating may occur at the nuclear membrane and not within the nucleus. Quantitative analysis demonstrated inefficiency in a number of steps including virus uptake and internalization, translocation of nucleocapsid across the nuclear membrane and antigen expression from intranuclear viral DNA. | - |
dc.language.iso | en | - |
dc.publisher | SOC GENERAL MICROBIOLOGY | - |
dc.source.uri | http://dx.doi.org/10.1099/0022-1317-80-8-2127 | - |
dc.subject | Liver | - |
dc.subject | Cells, Cultured | - |
dc.subject | Cell Nucleus | - |
dc.subject | Animals | - |
dc.subject | Ducks | - |
dc.subject | Hepatitis B Virus, Duck | - |
dc.subject | DNA, Viral | - |
dc.subject | Virus Replication | - |
dc.subject | DNA Replication | - |
dc.subject | Biological Transport | - |
dc.subject | Kinetics | - |
dc.title | Kinetics of early molecular events in duck hepatitis B virus replication in primary duck hepatocytes | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1099/0022-1317-80-8-2127 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Burrell, C. [0000-0002-4020-349X] | - |
Appears in Collections: | Aurora harvest 2 Microbiology and Immunology publications |
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