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https://hdl.handle.net/2440/11627
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DC Field | Value | Language |
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dc.contributor.author | Roberge, C. | - |
dc.contributor.author | McColl, S. | - |
dc.contributor.author | Larochelle, B. | - |
dc.contributor.author | Gosselin, J. | - |
dc.date.issued | 1998 | - |
dc.identifier.citation | Journal of Immunology, 1998; 160(5):2442-2448 | - |
dc.identifier.issn | 0022-1767 | - |
dc.identifier.issn | 1550-6606 | - |
dc.identifier.uri | http://hdl.handle.net/2440/11627 | - |
dc.description.abstract | We have recently demonstrated that EBV binds to human neutrophils and stimulates a wide range of activities, including homeotypic aggregation, total RNA synthesis, and expression of the chemokines IL-8 and macrophage inflammatory protein-1alpha (MIP-1alpha). Neutrophil function is also known to be modulated by priming with granulocyte-macrophage colony-stimulating factor (GM-CSF). We have therefore investigated the modulation of EBV-induced activation of human neutrophils by GM-CSF. Treatment of neutrophils with GM-CSF before EBV activation enhanced the production of both MIP-1alpha and IL-8. The IL-8 produced under these conditions was biologically active as determined in the calcium mobilization assay. GM-CSF was also found to increase the ability of EBV to prime neutrophils for increased leukotriene B4 (LTB4) synthesis. Prior treatment of GM-CSF with neutralizing Abs inhibited these effects. GM-CSF also increased the specific binding of FITC-EBV to the neutrophil surface, as evaluated by fluorocytometry. Local production of GM-CSF in tissues invaded by EBV could therefore serve to potentiate a host defense mechanism directed toward the destruction of the infectious virus via increased production of chemotactic factors. Since both IL-8 and MIP-1alpha are reported to be chemoattractants in vitro for T cells and T and B cells, respectively, the ability of EBV to induce their production by neutrophils may enhance its ability to infect B and T lymphocytes via increased recruitment to sites of infection. | - |
dc.language.iso | en | - |
dc.publisher | AMER ASSOC IMMUNOLOGISTS | - |
dc.subject | Neutrophils | - |
dc.subject | Humans | - |
dc.subject | Herpesvirus 4, Human | - |
dc.subject | Calcium | - |
dc.subject | Leukotriene B4 | - |
dc.subject | Granulocyte-Macrophage Colony-Stimulating Factor | - |
dc.subject | RNA, Messenger | - |
dc.subject | Interleukin-8 | - |
dc.subject | Macrophage Inflammatory Proteins | - |
dc.subject | Chemotactic Factors | - |
dc.subject | Adjuvants, Immunologic | - |
dc.subject | Neutrophil Activation | - |
dc.subject | Chemokine CCL3 | - |
dc.subject | Chemokine CCL4 | - |
dc.title | Granulocyte-macrophage colony-stimulating factor enhances EBV-induced synthesis of chemotactic factors in human neutrophils. | - |
dc.type | Journal article | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | McColl, S. [0000-0003-0949-4660] | - |
Appears in Collections: | Aurora harvest 2 Microbiology and Immunology publications |
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