Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/117621
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Type: | Journal article |
Title: | Characterization of the novel spontaneously immortalized rat Müller cell line SIRMu-1 |
Author: | Kittipassorn, T. Haydinger, C.D. Wood, J.P.M. Mammone, T. Casson, R.J. Peet, D.J. |
Citation: | Experimental Eye Research, 2019; 181:127-135 |
Publisher: | Elsevier |
Issue Date: | 2019 |
ISSN: | 0014-4835 1096-0007 |
Statement of Responsibility: | Thaksaon Kittipassorn, Cameron D. Haydinger, John P.M. Wood, Teresa Mammone, Robert J. Casson, Daniel J. Peet |
Abstract: | Müller cells (MCs) play a crucial role in the retina, and cultured MC lines are an important tool with which to study MC function. Transformed MC lines have been widely used; however, the transformation process can also lead to unwanted changes compared to the primary cells from which they were derived. To provide an alternative experimental tool, a monoclonal novel spontaneously immortalized rat Müller cell line, SIRMu-1, was derived from primary rat MCs and characterized. Immunofluorescence, western blotting and RNA sequencing demonstrate that the SIRMu-1 cell line retains similar characteristics to cultured primary MCs in terms of expression of the MC markers cellular retinaldehyde-binding protein, glutamine synthetase, S100, vimentin and glial fibrillary acidic protein at both the mRNA and protein levels. Both the cellular morphology and overall transcriptome of the SIRMu-1 cells are more similar to primary rat MCs than the commonly used rMC-1 cells, a well-described, transformed rat MC line. Furthermore, SIRMu-1 cells proliferate rapidly, have an effectively indefinite life span and a high transfection efficiency. The expression of Y chromosome specific genes confirmed that the SIRMu-1 cells are derived from male MCs. Thus, the SIRMu-1 cell line represents a valuable experimental tool to study roles of MCs in both physiological and pathological states. |
Keywords: | Cell culture Müller cell Retina SIRMu-1 Spontaneously immortalized cell |
Rights: | © 2019 Elsevier Ltd. All rights reserved. |
DOI: | 10.1016/j.exer.2019.01.013 |
Grant ID: | http://purl.org/au-research/grants/nhmrc/1099932 |
Published version: | http://dx.doi.org/10.1016/j.exer.2019.01.013 |
Appears in Collections: | Aurora harvest 8 Biochemistry publications |
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hdl_117621.pdf | Submitted version | 2.25 MB | Adobe PDF | View/Open |
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