Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/117830
Citations | ||
Scopus | Web of Science® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | Responsive upconversion nanoprobe for background-free hypochlorous acid detection and bioimaging |
Author: | Zhang, R. Liang, L. Meng, Q. Zhao, J. Ta, H.T. Li, L. Zhang, Z. Sultanbawa, Y. Xu, Z.P. |
Citation: | Small, 2019; 15(2):1803712-1-1803712-11 |
Publisher: | Wiley |
Issue Date: | 2019 |
ISSN: | 1613-6810 1613-6829 |
Statement of Responsibility: | Run Zhang, Liuen Liang, Qingtao Meng, Jiangbo Zhao, Hang T. Ta, Li Li, Zhiqiang Zhang, Yasmina Sultanbawa and Zhi Ping Xu |
Abstract: | Responsive nanoprobes play an important role in bioassay and bioimaging, early diagnosis of diseases and treatment monitoring. Herein, a upconversional nanoparticle (UCNP)-based nanoprobe, Ru@UCNPs, for specific sensing and imaging of hypochlorous acid (HOCl) is reported. This Ru@UCNP nanoprobe consists of two functional components,, i.e., NaYF₄ :Yb, Tm UCNPs that can convert near infrared light-to-visible light as the energy donor, and a HOCl-responsive ruthenium(II) complex [Ru(bpy)₂ (DNCH-bpy)](PF₆)₂ (Ru-DNPH) as the energy acceptor and also the upconversion luminescence (UCL) quencher. Within this luminescence resonance energy transfer nanoprobe system, the UCL OFF-ON emission is triggered specifically by HOCl. This triggering reaction enables the detection of HOCl in aqueous solution and biological systems. As an example of applications, the Ru@UCNPs nanoprobe is loaded onto test papers for semiquantitative HOCl detection without any interference from the background fluorescence. The application of Ru@UCNPs for background-free detection and visualization of HOCl in cells and mice is successfully demonstrated. This research has thus shown that Ru@UCNPs is a selective HOCl-responsive nanoprobe, providing a new way to detect HOCl and a new strategy to develop novel nanoprobes for in situ detection of various biomarkers in cells and early disgnosis of animal diseases. |
Keywords: | Bioassay and imaging; luminescence resonance energy transfer; nanoprobes; paper-based test strips; selective HOCl detection and imaging |
Rights: | © 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim |
DOI: | 10.1002/smll.201803712 |
Grant ID: | http://purl.org/au-research/grants/arc/DP170104643 http://purl.org/au-research/grants/arc/DE170100092 http://purl.org/au-research/grants/nhmrc/1125794 |
Published version: | http://dx.doi.org/10.1002/smll.201803712 |
Appears in Collections: | Aurora harvest 8 IPAS publications |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.