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https://hdl.handle.net/2440/121602
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Type: | Journal article |
Title: | Transcriptional variation is associated with differences in shoot sodium accumulation in distinct barley varieties |
Author: | Amarasinghe, S.L. Watson-Haigh, N.S. Byrt, C. James, R. Qiu, J. Berkowitz, O. Whelan, J. Roy, S.J. Gilliham, M. Baumann, U. |
Citation: | Environmental and Experimental Botany, 2019; 166:103812-1-103812-15 |
Publisher: | Elsevier |
Issue Date: | 2019 |
ISSN: | 0098-8472 1873-7307 |
Statement of Responsibility: | Shanika L. Amarasinghe, Nathan S. Watson-Haigh, Caitlin Byrt, Richard James, Jiaen Qiu, Oliver Berkowitz, James Whelan, Stuart J. Roy, Matthew Gilliham, Ute Baumann |
Abstract: | Soil salinity causes large productivity losses for agriculture worldwide. Barley has been identified as one of the more salt tolerant staple crops compared to wheat and rice. Identification of genes and allelic variations underlying various salt tolerance mechanisms in barley will be a practical contribution towards the development of cereal lines with greater salinity tolerance. Here, RNA from six barley varieties with varying leaf blade and sheath Na⁺ accumulation following salt (NaCl) treatment were sequenced. Differential gene expression analysis, variant calling and gene co-regulatory network analysis was conducted to identify potential molecular components underlying the shoot Na⁺ phenotypes. We identified novel alleles of HKT1;5 that could be responsible for high Na⁺ accumulation in blade and sheath. Furthermore, through statistical modelling of gene expression, a Na⁺/H⁺ Exchanger (NHX) gene was identified as a candidate for high sheath Na⁺ accumulation. Through co-expression networks, we discovered expression pattern variation for genes related to terpenoid phenylpropanoid and flavonoid metabolism amongst the six varieties. The gene candidates identified in this study provide us with targets of interest for future characterisation of molecular mechanisms that may contribute to salt stress tolerance in barley. |
Keywords: | Hordeum vulgare cultivars; HKT; NHX |
Rights: | Crown Copyright © 2019 Published by Elsevier B.V. All rights reserved. |
DOI: | 10.1016/j.envexpbot.2019.103812 |
Grant ID: | http://purl.org/au-research/grants/arc/CE140100008 http://purl.org/au-research/grants/arc/FT130100709 http://purl.org/au-research/grants/arc/DE150100837 |
Published version: | http://dx.doi.org/10.1016/j.envexpbot.2019.103812 |
Appears in Collections: | Agriculture, Food and Wine publications Aurora harvest 8 |
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