Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/124457
Citations | ||
Scopus | Web of Science® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | Structural characterization of the Pet c 1.0201 PR-10 protein isolated from roots of Petroselinum crispum (Mill.) Fuss |
Author: | Stratilova, B. Rehulka, P. Garajova, S. Rehulkova, H. Stratilova, E. Hrmova, M. Kozmon, S. |
Citation: | Phytochemistry: the international journal of plant chemistry, plant biochemistry and molecular biology, 2020; 175:112368-1-112368-9 |
Publisher: | Elsevier |
Issue Date: | 2020 |
ISSN: | 0031-9422 1873-3700 |
Statement of Responsibility: | Barbora Stratilová, Pavel Řehulka, Soňa Garajová, Helena Řehulková, Eva Stratilová, Maria Hrmova, Stanislav Kozmon |
Abstract: | The native dimeric Petroselinum crispum (Mill.) Fuss protein Pet c 1.0201 and a monomeric xyloglucan endotransglycosylase enzyme (Garajova et al., 2008) isolated from the root cells co-purify and share similar molecular masses and acidic isoelectric points. In this work, we determined the complete primary structure of the parsley Pet c 1.0201 protein, based on tryptic and chymotryptic peptides followed by the manual micro-gradient chromatographic separation coupled with offline MALDI-TOF/TOF mass spectrometry. The bioinformatics approach enabled us to include the parsley protein into the PR-10 family, as it exhibited the highest protein sequence identity with the Apium graveolens Api g 1.0201 allergen and the major Daucus carota allergen Dau c 1.0201. Hence, we designated the Petroselinum crispum protein as Pet c 1.0201 and deposited it in the UniProt Knowledgebase under the accession C0HKF5. 3D protein homology modelling and molecular dynamics simulations of the Pet c 1.0201 dimer confirmed the typical structure of the Bet v 1 family allergens, and the potential of the Pet c 1.0201 protein to dimerize in water. However, the behavioural properties of Pet c 1.0201 and the celery allergen Api g 1.0101 differed in the presence of salts due to transiently and stably formed dimeric forms of Pet c 1.0201 and Api g 1.0101, respectively. |
Keywords: | Petroselinum crispum; apiaceae; parsley; mass spectrometry; 3D structural modelling; molecular dynamics simulations; PR-10 proteins |
Rights: | © 2020 Elsevier Ltd. All rights reserved. |
DOI: | 10.1016/j.phytochem.2020.112368 |
Grant ID: | http://purl.org/au-research/grants/arc/DP120100900 |
Published version: | http://dx.doi.org/10.1016/j.phytochem.2020.112368 |
Appears in Collections: | Agriculture, Food and Wine publications Aurora harvest 8 |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
hdl_124457.pdf | Accepted version | 1.7 MB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.