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https://hdl.handle.net/2440/126558
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Type: | Journal article |
Title: | A label-free optical biosensor based on nanoporous anodic alumina for tumour necrosis factor-alpha detection in chronic wounds |
Author: | Rajeev, G. Xifre-Perez, E. Prieto Simon, B. Cowin, A.J. Marsal, L.F. Voelcker, N.H. |
Citation: | Sensors and Actuators B: Chemical: international journal devoted to research and development of physical and chemical transducers, 2018; 257:116-123 |
Publisher: | Elsevier |
Issue Date: | 2018 |
ISSN: | 0925-4005 |
Statement of Responsibility: | Gayathri Rajeev, Elisabet Xifre-Perez, Beatriz Prieto Simon, Allison J.Cowin, Lluis F.Marsal, Nicolas H.Voelcker |
Abstract: | Tumour necrosis factor-alpha (TNF-α) is a pro-inflammatory cytokine important to wound healing. In non-healing wounds, it has been suggested that the expression of TNF-α is prolonged and elevated which contributes to impaired healing responses. Hence it is of great interest to develop biosensors towards the detection of TNF-α in non-healing wounds. In this study, we have developed a label-free optical TNF-α biosensor based on interferometric reflectance spectroscopy (IRS) technique using a functionalized nanoporous anodic alumina (NAA) thin film transducer. The biosensor is fabricated by functionalizing NAA pore walls with anti-TNF-α antibodies using silanization chemistry. Binding of TNF-α to the bioreceptors within the pores causes a change in effective optical thickness (EOT) of the NAA thin film. Thus, analyte detection is achieved by monitoring EOT evolution with time. Label-free detection of TNF-α was demonstrated in buffer solution and in complex media such as simulated wound fluid. A limit of detection of 0.13 μg/mL was achieved. This study provides proof-of-concept evidence which sets foundations for further development of biosensors as point-of-care (POC) diagnostic tools for chronic wound care. |
Keywords: | Porous alumina biosensor; TNF-α detection; chronic wound; optical biosensor |
Rights: | © 2017 Elsevier B.V. All rights reserved. |
DOI: | 10.1016/j.snb.2017.10.156 |
Published version: | http://dx.doi.org/10.1016/j.snb.2017.10.156 |
Appears in Collections: | Aurora harvest 4 Chemistry publications |
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