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https://hdl.handle.net/2440/131530
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dc.contributor.author | Capon, P.K. | - |
dc.contributor.author | Horsfall, A.J. | - |
dc.contributor.author | Li, J. | - |
dc.contributor.author | Schartner, E.P. | - |
dc.contributor.author | Khalid, A. | - |
dc.contributor.author | Purdey, M.S. | - |
dc.contributor.author | McLaughlin, R.A. | - |
dc.contributor.author | Abell, A.D. | - |
dc.date.issued | 2021 | - |
dc.identifier.citation | RSC Advances: an international journal to further the chemical sciences, 2021; 11(36):22334-22342 | - |
dc.identifier.issn | 2046-2069 | - |
dc.identifier.issn | 2046-2069 | - |
dc.identifier.uri | http://hdl.handle.net/2440/131530 | - |
dc.description.abstract | We present a new coating procedure to prepare optical fibre sensors suitable for use with protein analytes. We demonstrate this through the detection of AlexaFluor-532 tagged streptavidin by its binding to D-biotin that is functionalised onto an optical fibre, via incorporation in a silk fibroin fibre coating. The D-biotin was covalently attached to a silk-binding peptide to provide SBP–biotin, which adheres the D-biotin to the silk-coated fibre tip. These optical fibre probes were prepared by two methods. The first involves dip-coating the fibre tip into a mixture of silk fibroin and SBP–biotin, which distributes the SBP–biotin throughout the silk coating (method A). The second method uses two steps, where the fibre is first dip-coated in silk only, then SBP–biotin added in a second dip-coating step. This isolates SBP–biotin to the outer surface of the silk layer (method B). A series of fluorescence measurements revealed that only the surface bound SBP–biotin detects streptavidin with a detection limit of 15 μg mL−1. The fibre coatings are stable to repeated washing and long-term exposure to water. Formation of silk coatings on fibres using commercial aqueous silk fibroin was found to be inhibited by a lithium concentration of 200 ppm, as determined by atomic absorption spectroscopy. This was reduced to less than 20 ppm by dialysis against water, and was found to successfully form a coating on optical fibres. | - |
dc.description.statementofresponsibility | Patrick K. Capon, Aimee J. Horsfall, Jiawen Li, Erik P. Schartner, Asma Khalid, Malcolm S. Purdey ... et al. | - |
dc.language.iso | en | - |
dc.publisher | Royal Society of Chemistry | - |
dc.rights | This journal is © The Royal Society of Chemistry 2021. | - |
dc.source.uri | http://dx.doi.org/10.1039/d1ra03584c | - |
dc.title | Protein detection enabled using functionalised silk-binding peptides on a silk-coated optical fibre | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1039/d1ra03584c | - |
dc.relation.grant | http://purl.org/au-research/grants/arc/CE140100003 | - |
dc.relation.grant | http://purl.org/au-research/grants/nhmrc/2001646 | - |
dc.relation.grant | http://purl.org/au-research/grants/nhmrc/102093 | - |
dc.relation.grant | http://purl.org/au-research/grants/arc/LP150100657 | - |
dc.relation.grant | http://purl.org/au-research/grants/nhmrc/1178912 | - |
dc.relation.grant | http://purl.org/au-research/grants/nhmrc/2002254 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Capon, P.K. [0000-0002-7396-5757] | - |
dc.identifier.orcid | Horsfall, A.J. [0000-0003-1276-2742] | - |
dc.identifier.orcid | Li, J. [0000-0001-8818-6070] | - |
dc.identifier.orcid | Schartner, E.P. [0000-0003-1669-4302] | - |
dc.identifier.orcid | Purdey, M.S. [0000-0002-5063-8972] | - |
dc.identifier.orcid | McLaughlin, R.A. [0000-0001-6947-5061] | - |
dc.identifier.orcid | Abell, A.D. [0000-0002-0604-2629] | - |
Appears in Collections: | Aurora harvest 4 Chemistry publications |
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