Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/133423
Citations | ||
Scopus | Web of Science® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | Transcript Profiling of MIKCc MADS-Box Genes Reveals Conserved and Novel Roles in Barley Inflorescence Development |
Author: | Kuijer, H.N.J. Shirley, N.J. Khor, S.F. Shi, J. Schwerdt, J. Zhang, D. Li, G. Burton, R.A. |
Citation: | Frontiers in Plant Science, 2021; 12:1-23 |
Publisher: | Frontiers Media SA |
Issue Date: | 2021 |
ISSN: | 1664-462X 1664-462X |
Statement of Responsibility: | Hendrik N. J. Kuijer, Neil J. Shirley, Shi F. Khor, Jin Shi, Julian Schwerdt, Dabing Zhang, Gang Li, and Rachel A. Burton |
Abstract: | MADS-box genes have a wide range of functions in plant reproductive development and grain production. The ABCDE model of floral organ development shows that MADS-box genes are central players in these events in dicotyledonous plants but the applicability of this model remains largely unknown in many grass crops. Here, we show that transcript analysis of all MIKCc MADS-box genes through barley (Hordeum vulgare L.) inflorescence development reveals co-expression groups that can be linked to developmental events. Thirty-four MIKCc MADS-box genes were identified in the barley genome and single-nucleotide polymorphism (SNP) scanning of 22,626 barley varieties revealed that the natural variation in the coding regions of these genes is low and the sequences have been extremely conserved during barley domestication. More detailed transcript analysis showed that MADS-box genes are generally expressed at key inflorescence developmental phases and across various floral organs in barley, as predicted by the ABCDE model. However, expression patterns of some MADS genes, for example HvMADS58 (AGAMOUS subfamily) and HvMADS34 (SEPALLATA subfamily), clearly deviate from predicted patterns. This places them outside the scope of the classical ABCDE model of floral development and demonstrates that the central tenet of antagonism between A- and C-class gene expression in the ABC model of other plants does not occur in barley. Co-expression across three correlation sets showed that specifically grouped members of the barley MIKCc MADS-box genes are likely to be involved in developmental events driving inflorescence meristem initiation, floral meristem identity and floral organ determination. Based on these observations, we propose a potential floral ABCDE working model in barley, where the classic model is generally upheld, but that also provides new insights into the role of MIKCc MADS-box genes in the developing barley inflorescence. |
Keywords: | barley inflorescence floral organs meristems MADS transcript profiling ABC model floret |
Rights: | Copyright © 2021 Kuijer, Shirley, Khor, Shi, Schwerdt, Zhang, Li and Burton. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
DOI: | 10.3389/fpls.2021.705286 |
Grant ID: | http://purl.org/au-research/grants/arc/DP170103267 http://purl.org/au-research/grants/arc/DP170103352 |
Published version: | http://dx.doi.org/10.3389/fpls.2021.705286 |
Appears in Collections: | Agriculture, Food and Wine publications |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
hdl_133423.pdf | 11.28 MB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.