Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/133478
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dc.contributor.author | Nefzger, C.M. | - |
dc.contributor.author | Jardé, T. | - |
dc.contributor.author | Rossello, F.J. | - |
dc.contributor.author | Horvay, K. | - |
dc.contributor.author | Knaupp, A.S. | - |
dc.contributor.author | Powell, D.R. | - |
dc.contributor.author | Chen, J. | - |
dc.contributor.author | Abud, H.E. | - |
dc.contributor.author | Polo, J.M. | - |
dc.date.issued | 2016 | - |
dc.identifier.citation | Stem Cell Reports, 2016; 6(3):321-329 | - |
dc.identifier.issn | 2213-6711 | - |
dc.identifier.issn | 2213-6711 | - |
dc.identifier.uri | https://hdl.handle.net/2440/133478 | - |
dc.description.abstract | The isolation of pure populations of mouse intestinal stem cells (ISCs) is essential to facilitate functional studies of tissue homeostasis, tissue regeneration, and intestinal diseases. However, the purification of ISCs has relied predominantly on the use of transgenic reporter alleles in mice. Here, we introduce a combinational cell surface marker-mediated strategy that allows the isolation of an ISC population transcriptionally and functionally equivalent to the gold standard Lgr5-GFP ISCs. Used on reporter-free mice, this strategy allows the isolation of functional, transcriptionally distinct ISCs uncompromised by Lgr5 haploinsufficiency. | - |
dc.description.statementofresponsibility | Christian M. Nefzger, Thierry Jardé, Fernando J.Rossello, Katja Horvay, Anja S.Knaupp, David R.Powell ... et al. | - |
dc.language.iso | en | - |
dc.publisher | Cell Press | - |
dc.rights | © 2016 The Authors. Published by Elsevier Inc. | - |
dc.source.uri | http://dx.doi.org/10.1016/j.stemcr.2016.01.014 | - |
dc.subject | Intestinal Mucosa | - |
dc.subject | Cells, Cultured | - |
dc.subject | Animals | - |
dc.subject | Mice, Inbred C57BL | - |
dc.subject | Mice | - |
dc.subject | Receptors, G-Protein-Coupled | - |
dc.subject | Male | - |
dc.subject | Adult Stem Cells | - |
dc.subject | Primary Cell Culture | - |
dc.subject.mesh | Intestinal Mucosa | - |
dc.subject.mesh | Cells, Cultured | - |
dc.subject.mesh | Animals | - |
dc.subject.mesh | Mice, Inbred C57BL | - |
dc.subject.mesh | Mice | - |
dc.subject.mesh | Receptors, G-Protein-Coupled | - |
dc.subject.mesh | Male | - |
dc.subject.mesh | Adult Stem Cells | - |
dc.subject.mesh | Primary Cell Culture | - |
dc.title | A versatile strategy for isolating a highly enriched population of intestinal stem cells | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1016/j.stemcr.2016.01.014 | - |
dc.relation.grant | http://purl.org/au-research/grants/nhmrc/1061883 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Polo, J.M. [0000-0002-2531-778X] | - |
Appears in Collections: | Medical Sciences publications |
Files in This Item:
File | Description | Size | Format | |
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hdl_133478.pdf | Published version | 2.26 MB | Adobe PDF | View/Open |
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