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https://hdl.handle.net/2440/135301
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Type: | Journal article |
Title: | Transmission of Klebsiella strains and plasmids within and between Grey-headed flying fox colonies |
Author: | Vezina, B. Judd, L.M. McDougall, F.K. Boardman, W.S.J. Power, M.L. Hawkey, J. Brisse, S. Monk, J.M. Holt, K.E. Wyres, K.L. |
Citation: | Environmental Microbiology, 2022; 24(9):4425-4436 |
Publisher: | Wiley |
Issue Date: | 2022 |
ISSN: | 1462-2912 1462-2920 |
Statement of Responsibility: | Ben Vezina, Louise M. Judd, Fiona K. McDougall, Wayne S. J. Boardman, Michelle L. Power, Jane Hawkey, Sylvain Brisse, Jonathan M. Monk, Kathryn E. Holt, and Kelly L. Wyres |
Abstract: | The grey-headed flying fox (Pteropus poliocephalus) is an endemic Australian fruit bat, known to carry zoonotic pathogens. We recently showed they harbour bacterial pathogen Klebsiella pneumoniae and closely related species in the K. pneumoniae species complex (KpSC); however, the dynamics of KpSC transmission and gene flow within flying fox colonies are poorly understood. High-resolution genome comparisons of 39 KpSC isolates from grey-headed flying foxes identified five putative strain transmission clusters (four intra- and one inter-colony). The instance of inter-colony strain transmission of K. africana was found between two flying fox populations within flying distance, indicating either direct or indirect transmission through a common food/water source. All 11 plasmids identified within the KpSC isolates showed 73% coverage (mean) and ≥95% identity to human-associated KpSC plasmids, indicating gene flow between human clinical and grey-headed flying fox isolates. Along with strain transmission, inter-species horizontal plasmid transmission between K. pneumoniae and Klebsiella africana was also identified within a flying fox colony. Finally, genome-scale metabolic models were generated to predict and compare substrate usage to previously published KpSC models, from human and environmental sources. These models indicated no distinction on the basis of metabolic capabilities. Instead, metabolic capabilities were consistent with population structure and ST/lineage. |
Keywords: | Animals Chiroptera Humans Klebsiella Water Plasmids Australia |
Description: | First published: 19 May 2022 |
Rights: | © 2022 The Authors. Environmental Microbiology published by Society for Applied Microbiology and John Wiley & Sons Ltd.This is an open access article under the terms of the Creative Commons AttributionLicense, which permits use, distribution andreproduction in any medium, provided the original work is properly cited. |
DOI: | 10.1111/1462-2920.16047 |
Grant ID: | http://purl.org/au-research/grants/arc/DP200103364 http://purl.org/au-research/grants/nhmrc/1176192 |
Published version: | http://dx.doi.org/10.1111/1462-2920.16047 |
Appears in Collections: | Animal and Veterinary Sciences publications |
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hdl_135301.pdf | Published version | 1.95 MB | Adobe PDF | View/Open |
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