Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/135457
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dc.contributor.author | Leinenga, G. | - |
dc.contributor.author | Bodea, L.G. | - |
dc.contributor.author | Schröder, J. | - |
dc.contributor.author | Sun, G. | - |
dc.contributor.author | Zhou, Y. | - |
dc.contributor.author | Song, J. | - |
dc.contributor.author | Grubman, A. | - |
dc.contributor.author | Polo, J.M. | - |
dc.contributor.author | Götz, J. | - |
dc.date.issued | 2023 | - |
dc.identifier.citation | Bioengineering & Translational Medicine, 2023; 8(1):e10329-1-e10329-14 | - |
dc.identifier.issn | 2380-6761 | - |
dc.identifier.issn | 2380-6761 | - |
dc.identifier.uri | https://hdl.handle.net/2440/135457 | - |
dc.description | First published: 03 May 2022 | - |
dc.description.abstract | Transcranial scanning ultrasound combined with intravenously injected microbubbles(SUS⁺MB) has been shown to transiently open the blood–brain barrier and reduce the amyloid-β(Aβ) pathology in the APP23 mouse model of Alzheimer's disease (AD). This has been accomplished through the activation of microglial cells; however, their response to the SUS treatment is incompletely understood. Here, wild-type (WT) and APP23 mice were subjected to SUS⁺MB, using nonsonicated mice as sham controls. After 48 h, the APP23 mice were injected with methoxy-XO4 to label Aβaggregates, followed by microglial isolation into XO4⁺ and XO4‾ populations using flow cytometry. Both XO4⁺ and XO4‾ cells were subjected to RNA sequencing and transcriptome pro-filing. The analysis of the microglial cells revealed a clear segregation depending on genotype (AD model vs. WT mice) and Aβinternalization (XO4⁺ vs. XO4‾ microglia),but interestingly, no differences were found between SUS⁺ MB and sham in WT mice. Differential gene expression analysis in APP23 mice detected 278 genes that were significantly changed by SUS⁺ MB in the XO4⁺ cells (248 up/30 down) and 242 in XO‾ cells(225 up/17 down). Pathway analysis highlighted differential expression of genes related to the phagosome pathway and marked upregulation of cell cycle-related transcripts in XO4⁺ and XO4- microglia isolated from SUS⁺MB-treated APP23 mice. Together, this highlights the complexity of the microglial response to transcranial ultrasound, with potential applications for the treatment of AD. | - |
dc.description.statementofresponsibility | Gerhard Leinenga, Liviu-Gabriel Bodea, Jan Schröder, Giuzhi Sun, Yichen Zhou, Jae Song, Alexandra Grubman, Jose M. Polo, Jürgen Götz | - |
dc.language.iso | en | - |
dc.publisher | Wiley | - |
dc.rights | © 2022 The Authors. Bioengineering & Translational Medicine published by Wiley Periodicals LLC on behalf of American Institute of Chemical Engineers. | - |
dc.source.uri | http://dx.doi.org/10.1002/btm2.10329 | - |
dc.subject | Alzheimer's disease | - |
dc.subject | RNA sequencing | - |
dc.subject | methoxy‐XO4 | - |
dc.subject | microglia | - |
dc.subject | transcriptomics | - |
dc.subject | ultrasound | - |
dc.title | Transcriptional signature in microglia isolated from an Alzheimer's disease mouse model treated with scanning ultrasound | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1002/btm2.10329 | - |
dc.relation.grant | http://purl.org/au-research/grants/nhmrc/GNT1145580 | - |
dc.relation.grant | http://purl.org/au-research/grants/nhmrc/GNT1176326 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Polo, J.M. [0000-0002-2531-778X] | - |
Appears in Collections: | Medical Sciences publications |
Files in This Item:
File | Description | Size | Format | |
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hdl_135457.pdf | Published version | 4.25 MB | Adobe PDF | View/Open |
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