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https://hdl.handle.net/2440/135582
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Type: | Journal article |
Title: | Enhanced reactive oxygen detoxification occurs in salt-stressed soybean roots expressing GmSALT3 |
Author: | Qu, Y. Guan, R. Yu, L. Berkowitz, O. David, R. Whelan, J. Ford, M. Wege, S. Qiu, L. Gilliham, M. |
Citation: | Physiologia Plantarum, 2022; 174(3):1-16 |
Publisher: | Wiley |
Issue Date: | 2022 |
ISSN: | 0031-9317 1399-3054 |
Statement of Responsibility: | Yue Qu, Rongxia Guan, Lili Yu, Oliver Berkowitz, Rakesh David, James Whelan, Melanie Ford, Stefanie Wege, Lijuan Qiu, Matthew Gilliham |
Abstract: | Soybean (Glycine max) is an important crop globally for food and edible oil production. Soybean plants are sensitive to salinity (NaCl), with significant yield decreases reported under saline conditions.GmSALT3is the dominant gene underlying a major QTL for salt tolerance in soybean.GmSALT3encodes a transmembrane protein belonging to the plant cation/proton exchanger (CHX) family, and is predominately expressed in root phloem and xylem associated cells under both saline and non-saline conditions. It is currently unknown through which molecular mechanism(s) the ER-localised GmSALT3 contributes to salinity tolerance, as its localisation excludes direct involvement in ion exclusion. In order to gain insights into potential molecula rmechanism(s), we used RNA-seq analysis of roots from two soybean NILs (near isogenic lines); NIL-S (salt-sensitive,Gmsalt3), and NIL-T (salt-tolerant,GmSALT3), grown under control and saline conditions (200 mM NaCl) at three time points (0 h, 6 h, and3 days). Gene ontology (GO) analysis showed that NIL-T has greater responses aligned to oxidation reduction. ROS were less abundant and scavenging enzyme activity was greater in NIL-T, consistent with the RNA-seq data. Further analysis indicated that genes related to calcium signalling, vesicle trafficking and Casparian strip(CS) development were upregulated in NIL-T following salt treatment. We propose that GmSALT3 improves the ability of NIL-T to cope with saline stress through preventing ROS over accumulation in roots, and potentially modulating Ca2+signal-ling, vesicle trafficking and formation of diffusion barriers. |
Keywords: | Fabaceae Soybeans Plant Roots Sodium Chloride Oxygen Reactive Oxygen Species Gene Expression Regulation, Plant Salt Tolerance |
Rights: | © 2022 The Authors.Physiologia Plantarumpublished by John Wiley & Sons Ltd on behalf of Scandinavian Plant Physiology Society. This is an open access article under the terms of theCreative Commons Attribution-NonCommercialLicense, which permits use, distribution and reproduction in anymedium, provided the original work is properly cited and is not used for commercial purposes. |
DOI: | 10.1111/ppl.13709 |
Grant ID: | http://purl.org/au-research/grants/arc/CE140100008 http://purl.org/au-research/grants/arc/DE160100804 http://purl.org/au-research/grants/arc/FT130100709 |
Published version: | http://dx.doi.org/10.1111/ppl.13709 |
Appears in Collections: | Agriculture, Food and Wine publications |
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hdl_135582.pdf | Published version | 20.32 MB | Adobe PDF | View/Open |
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