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https://hdl.handle.net/2440/135788
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Type: | Journal article |
Title: | Transforming growth factor β1 impairs the transcriptomic response to contraction in myotubes from women with polycystic ovary syndrome |
Other Titles: | Transforming growth factor beta 1 impairs the transcriptomic response to contraction in myotubes from women with polycystic ovary syndrome |
Author: | McIlvenna, L.C. Altıntaş, A. Patten, R.K. McAinch, A.J. Rodgers, R.J. Stepto, N.K. Barrès, R. Moreno-Asso, A. |
Citation: | The Journal of Physiology, 2022; 600(14):3313-3330 |
Publisher: | Wiley |
Issue Date: | 2022 |
ISSN: | 0022-3751 1469-7793 |
Statement of Responsibility: | Luke C. McIlvenna, Ali Altıntas, Rhiannon K. Patten, Andrew J. McAinch, Raymond J. Rodgers, Nigel K. Stepto, Romain Barrès, and Alba Moreno-Asso |
Abstract: | Polycystic ovary syndrome (PCOS) is characterised by a hormonal imbalance affecting the reproductive and metabolic health of reproductive-aged women. Exercise is recommended as a first-line therapy for women with PCOS to improve their overall health; however, women with PCOSare resistant to the metabolic benefits of exercise training. Here, we aimed to gain insight into the mechanisms responsible for such resistance to exercise in PCOS. We employed an in vitro approach with electrical pulse stimulation (EPS) of cultured skeletal muscle cells to explore whether myotubes from women with PCOS have an altered gene expression signature in response to contraction.Following EPS, 4719 genes were differentially expressed (false discovery rate<0.05) in myotubes from women with PCOS compared to 173 in healthy women. Both groups included genes involved in skeletal muscle contraction. We also determined the effect of two transforming growth factorβ(TGFβ) ligands that are elevated in plasma of women with PCOS, TGFβ1 and anti-Müllerianhormone (AMH), alone and on the EPS-induced response. While AMH (30 ng/ml) had no effect,TGFβ1 (5 ng/ml) induced the expression of extracellular matrix genes and impaired the exercise-like transcriptional signature in myotubes from women with and without PCOS in response to EPS by interfering with key processes related to muscle contraction, calcium transport and actin filament.Our findings suggest that while the fundamental gene expression responses of skeletal muscle to contraction is intact in PCOS, circulating factors like TGFβ1 may be responsible for the impaired adaptation to exercise in women with PCOS. |
Keywords: | Humans Polycystic Ovary Syndrome Adult Female Transforming Growth Factor beta1 Anti-Mullerian Hormone Muscle Fibers, Skeletal Transcriptome |
Description: | First published online 27 June 2022 |
Rights: | © 2022 The Authors. The Journal of Physiology published by John Wiley & Sons Ltd on behalf of The Physiological Society.This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
DOI: | 10.1113/JP282954 |
Published version: | http://dx.doi.org/10.1113/jp282954 |
Appears in Collections: | Obstetrics and Gynaecology publications |
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File | Description | Size | Format | |
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hdl_135788.pdf | Published version | 2.88 MB | Adobe PDF | View/Open |
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