Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/137315
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DC Field | Value | Language |
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dc.contributor.author | Delon, L.C. | - |
dc.contributor.author | Faria, M. | - |
dc.contributor.author | Jia, Z. | - |
dc.contributor.author | Johnston, S. | - |
dc.contributor.author | Gibson, R. | - |
dc.contributor.author | Prestidge, C.A. | - |
dc.contributor.author | Thierry, B. | - |
dc.date.issued | 2023 | - |
dc.identifier.citation | Small Methods, 2023; 7(1):1-12 | - |
dc.identifier.issn | 2366-9608 | - |
dc.identifier.issn | 2366-9608 | - |
dc.identifier.uri | https://hdl.handle.net/2440/137315 | - |
dc.description.abstract | Understanding the intestinal transport of particles is critical in several fields ranging from optimizing drug delivery systems to capturing health risks from the increased presence of nano- and micro-sized particles in human environment. While Caco-2 cell monolayers grown on permeable supports are the traditional in vitro model used to probe intestinal absorption of dis-solved molecules, they fail to recapitulate the transcytotic activity of polar-ized enterocytes. Here, an intestine-on-chip model is combined with in silico modeling to demonstrate that the rate of particle transcytosis is ≈350× higher across Caco-2 cell monolayers exposed to fluid shear stress compared to Caco-2 cells in standard “static” configuration. This relates to profound phe-notypical alterations and highly polarized state of cells grown under mechan-ical stimulation and it is shown that transcytosis in the microphysiological model is energy-dependent and involves both clathrin and macropinocytosis mediated endocytic pathways. Finally, it is demonstrated that the increased rate of transcytosis through cells exposed to flow is explained by a higher rate of internal particle transport (i.e., vesicular cellular trafficking and baso-lateral exocytosis), rather than a change in apical uptake (i.e., binding and endocytosis). Taken together, the findings have important implications for addressing research questions concerning intestinal transport of engineered and environmental particles. | - |
dc.description.statementofresponsibility | Ludivine C. Delon, Matthew Faria, Zhengyang Jia, Stuart Johnston, Rachel Gibson, Clive A. Prestidge, and Benjamin Thierry | - |
dc.language.iso | en | - |
dc.publisher | Wiley | - |
dc.rights | © 2022 The Authors. Small Methods published by Wiley-VCH GmbH. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purpose. | - |
dc.source.uri | http://dx.doi.org/10.1002/smtd.202200989 | - |
dc.subject | cellular transcytosis | - |
dc.subject | enterocytes | - |
dc.subject | intestinal absorption | - |
dc.subject | intestine-on-chip | - |
dc.title | Capturing and Quantifying Particle Transcytosis with Microphysiological Intestine-on-Chip Models | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1002/smtd.202200989 | - |
dc.relation.grant | http://purl.org/au-research/grants/arc/LP150100032 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Gibson, R. [0000-0002-4796-1621] | - |
Appears in Collections: | Molecular and Biomedical Science publications |
Files in This Item:
File | Description | Size | Format | |
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hdl_137315.pdf | Published version | 2.28 MB | Adobe PDF | View/Open |
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