Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/138316
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Type: | Journal article |
Title: | Mitochondrial DNA Supplementation of Oocytes Has Downstream Effects on the Transcriptional Profiles of Sus scrofa Adult Tissues with High mtDNA Copy Number |
Author: | Okada, T. Penn, A. St. John, J.C. |
Citation: | International Journal of Molecular Sciences, 2023; 24(8):7545-7545 |
Publisher: | MDPI AG |
Issue Date: | 2023 |
ISSN: | 1661-6596 1422-0067 |
Statement of Responsibility: | Takashi Okada, Alexander Penn and Justin C. St. John |
Abstract: | Oocytes can be supplemented with extra copies of mitochondrial DNA (mtDNA) to enhance developmental outcome. Pigs generated through supplementation with mtDNA derived from either sister (autologous) or third-party (heterologous) oocytes have been shown to exhibit only minor differences in growth, physiological and biochemical assessments, and health and well-being do not appear affected. However, it remains to be determined whether changes in gene expression identified during preimplantation development persisted and affected the gene expression of adult tissues indicative of high mtDNA copy number. It is also unknown if autologous and heterologous mtDNA supplementation resulted in different patterns of gene expression. Our transcriptome analyses revealed that genes involved in immune response and glyoxylate metabolism were commonly affected in brain, heart and liver tissues by mtDNA supplementation. The source of mtDNA influenced the expression of genes associated with oxidative phosphorylation (OXPHOS), suggesting a link between the use of third-party mtDNA and OXPHOS. We observed a significant difference in parental allelespecific imprinted gene expression in mtDNA-supplemented-derived pigs, with shifts to biallelic expression with no effect on expression levels. Overall, mtDNA supplementation influences the expression of genes in important biological processes in adult tissues. Consequently, it is important to determine the effect of these changes on animal development and health. |
Keywords: | autologous; heterologous; glyoxylate metabolism; immune response; imprinted gene; mitochondrial DNA; mitochondrial DNA supplementation; oxidative phosphorylation; Sus scrofa; transcriptome analysis |
Rights: | © 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/). |
DOI: | 10.3390/ijms24087545 |
Grant ID: | http://purl.org/au-research/grants/nhmrc/GNT1136065 http://purl.org/au-research/grants/nhmrc/GNT1160106 http://purl.org/au-research/grants/nhmrc/GNT2000723 |
Published version: | http://dx.doi.org/10.3390/ijms24087545 |
Appears in Collections: | Molecular and Biomedical Science publications |
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hdl_138316.pdf | Published version | 2.83 MB | Adobe PDF | View/Open |
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