Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/138723
Citations | ||
Scopus | Web of Science® | Altmetric |
---|---|---|
?
|
?
|
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Li, X. | - |
dc.contributor.author | Gong, P. | - |
dc.contributor.author | Zhou, X. | - |
dc.contributor.author | Wang, S. | - |
dc.contributor.author | Liu, Y. | - |
dc.contributor.author | Zhang, Y. | - |
dc.contributor.author | Nguyen, L.V. | - |
dc.contributor.author | Warren-Smith, S.C. | - |
dc.contributor.author | Zhao, Y. | - |
dc.date.issued | 2023 | - |
dc.identifier.citation | Analytica Chimica Acta, 2023; 1263:341286-1-341286-8 | - |
dc.identifier.issn | 0003-2670 | - |
dc.identifier.issn | 1873-4324 | - |
dc.identifier.uri | https://hdl.handle.net/2440/138723 | - |
dc.description.abstract | An advanced multi-parameter optical fiber sensing technology for EGFR gene detection based on DNA hybridization technology is demonstrated in this paper. For traditional DNA hybridization detection methods, temperature and pH compensation can not be realized or need multiple sensor probes. However, the multi-parameter detection technology we proposed can simultaneously detect complementary DNA, temperature and pH based on a single optical fiber probe. In this scheme, three optical signals including dual surface plasmon resonance signal (SPR) and Mach-Zehnder interference signal (MZI) are excited by binding the probe DNA sequence and pHsensitive material with the optical fiber sensor. The paper proposes the first research to achieve simultaneous excitation of dual SPR signal and Mach-Zehnder interference signal in a single fiber and used for three-parameter detection. Three optical signals have different sensitivities to the three variables. From a mathematical point of view, the unique solutions of exon-20 concentration, temperature and pH can be obtained by analyzing the three optical signals. The experimental results show that the exon-20 sensitivity of the sensor can reach 0.07 nm nM− 1 , and the limit of detection is 3.27 nM. The designed sensor gives a fast response, high sensitivity, and low detection limit, which is important for the field of DNA hybridization research and for solving the problems of biosensor susceptibility to temperature and pH. | - |
dc.description.statementofresponsibility | Xuegang Li, Pengqi Gong, Xue Zhou, Shankun Wang, Yingxuan Liu, Yanan Zhang, Linh V. Nguyen, Stephen C. Warren-Smith, Yong Zhao | - |
dc.language.iso | en | - |
dc.publisher | Elsevier BV | - |
dc.rights | © 2023 Elsevier B.V. All rights reserved. | - |
dc.source.uri | http://dx.doi.org/10.1016/j.aca.2023.341286 | - |
dc.subject | Biosensors; DNA sensor; Three parameter detection; Optical fiber; SPR; MZI | - |
dc.subject.mesh | DNA, Complementary | - |
dc.subject.mesh | Surface Plasmon Resonance | - |
dc.subject.mesh | Temperature | - |
dc.subject.mesh | Genes, erbB-1 | - |
dc.subject.mesh | Hydrogen-Ion Concentration | - |
dc.subject.mesh | Optical Fibers | - |
dc.title | In-situ detection scheme for EGFR gene with temperature and pH compensation using a triple-channel optical fiber biosensor | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1016/j.aca.2023.341286 | - |
dc.relation.grant | http://purl.org/au-research/grants/arc/CE14010003 | - |
dc.relation.grant | http://purl.org/au-research/grants/arc/FT200100154 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Nguyen, L.V. [0000-0002-2543-2374] | - |
dc.identifier.orcid | Warren-Smith, S.C. [0000-0002-2612-6344] | - |
Appears in Collections: | Physics publications |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.