Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/139336
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dc.contributor.author | Dwapanyin, G.O. | - |
dc.contributor.author | Chow, D.J.X. | - |
dc.contributor.author | Tan, T.C.Y. | - |
dc.contributor.author | Dubost, N.S. | - |
dc.contributor.author | Morizet, J.M. | - |
dc.contributor.author | Dunning, K.R. | - |
dc.contributor.author | Dholakia, K. | - |
dc.date.issued | 2023 | - |
dc.identifier.citation | Biomedical Optics Express, 2023; 14(7):3327-3342 | - |
dc.identifier.issn | 2156-7085 | - |
dc.identifier.issn | 2156-7085 | - |
dc.identifier.uri | https://hdl.handle.net/2440/139336 | - |
dc.description.abstract | Embryo quality is a crucial factor affecting live birth outcomes. However, an accurate diagnostic for embryo quality remains elusive in the in vitro fertilization clinic. Determining physical parameters of the embryo may offer key information for this purpose. Here, we demonstrate that digital holographic microscopy (DHM) can rapidly and non-invasively assess the refractive index of mouse embryos. Murine embryos were cultured in either low- or high-lipid containing media and digital holograms recorded at various stages of development. The phase of the recorded hologram was numerically retrieved, from which the refractive index of the embryo was calculated. We showed that DHM can detect spatio-temporal changes in refractive index during embryo development that are reflective of its lipid content. As accumulation of intracellular lipid is known to compromise embryo health, DHM may prove beneficial in developing an accurate, non-invasive, multimodal diagnostic. | - |
dc.description.statementofresponsibility | George O. Dwapanyin, Darren J. X. Chow, Tiffany C. Y. Tan, Nicolas S. Dubost, Josephine M. Morizet, Kylie R. Dunning, and Kishan Dholakia | - |
dc.language.iso | en | - |
dc.publisher | Optical Society of America | - |
dc.rights | © 2023 Optica Publishing Group under the terms of the Creative Commons Attribution 4.0 License. Further distribution of this work must maintain attribution to the author(s) and the published article's title, journal citation, and DOI. | - |
dc.source.uri | http://dx.doi.org/10.1364/boe.492292 | - |
dc.title | Investigation of refractive index dynamics during in vitro embryo development using off-axis digital holographic microscopy | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1364/BOE.492292 | - |
dc.relation.grant | http://purl.org/au-research/grants/arc/FL210100099 | - |
dc.relation.grant | http://purl.org/au-research/grants/nhmrc/2003786 | - |
pubs.publication-status | Published online | - |
dc.identifier.orcid | Dunning, K.R. [0000-0002-0462-6479] | - |
dc.identifier.orcid | Dholakia, K. [0000-0001-6534-9009] | - |
Appears in Collections: | IPAS publications |
Files in This Item:
File | Description | Size | Format | |
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hdl_139336.pdf | Published version | 7.21 MB | Adobe PDF | View/Open |
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