Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/140761
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Type: | Journal article |
Title: | Induction of muscle-regenerative multipotent stem cells from human adipocytes by PDGF-AB and 5-azacytidine |
Author: | Yeola, A. Subramanian, S. Oliver, R.A. Lucas, C.A. Thoms, J.A.I. Yan, F. Olivier, J. Chacon, D. Tursky, M.L. Srivastava, P. Potas, J.R. Hung, T. Power, C. Hardy, P. Ma, D.D. Kilian, K.A. McCarroll, J. Kavallaris, M. Hesson, L.B. Beck, D. et al. |
Citation: | Science Advances, 2021; 7(3):eabd1929-1-eabd1929-12 |
Publisher: | American Association for the Advancement of Science (AAAS) |
Issue Date: | 2021 |
ISSN: | 2375-2548 2375-2548 |
Statement of Responsibility: | Avani Yeola ... Vashe Chandrakanthan ... et al. |
Abstract: | Terminally differentiated murine osteocytes and adipocytes can be reprogrammed using platelet-derived growth factor-AB and 5-azacytidine into multipotent stem cells with stromal cell characteristics. We have now optimized culture conditions to reprogram human adipocytes into induced multipotent stem (iMS) cells and characterized their molecular and functional properties. Although the basal transcriptomes of adipocyte-derived iMS cells and adipose tissue-derived mesenchymal stem cells were similar, there were changes in histone modifications and CpG methylation at cis-regulatory regions consistent with an epigenetic landscape that was primed for tissue development and differentiation. In a non-specific tissue injury xenograft model, iMS cells contributed directly to muscle, bone, cartilage, and blood vessels, with no evidence of teratogenic potential. In a cardiotoxin muscle injury model, iMS cells contributed specifically to satellite cells and myofibers without ectopic tissue formation. Together, human adipocyte-derived iMS cells regenerate tissues in a context-dependent manner without ectopic or neoplastic growth. |
Keywords: | Adipocytes Adipose Tissue Animals Azacitidine Cell Differentiation Cells, Cultured Humans Mice Multipotent Stem Cells Muscles Platelet-Derived Growth Factor |
Rights: | © 2021 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S.Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). |
DOI: | 10.1126/sciadv.abd1929 |
Grant ID: | http://purl.org/au-research/grants/nhmrc/1119152 http://purl.org/au-research/grants/arc/FT180100417 http://purl.org/au-research/grants/nhmrc/1091261 http://purl.org/au-research/grants/nhmrc/1139811 |
Published version: | http://dx.doi.org/10.1126/sciadv.abd1929 |
Appears in Collections: | Research Outputs |
Files in This Item:
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hdl_140761.pdf | Published version | 12.55 MB | Adobe PDF | View/Open |
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