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https://hdl.handle.net/2440/14466
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Type: | Journal article |
Title: | Determination of perhexiline and hydroxyperhexiline in plasma by liquid chromatography-mass spectrometry |
Author: | Beck, O. Stephanson, N. Morris, R. Sallustio, B. Hjemdahl, P. |
Citation: | Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 2004; 805(1):87-91 |
Publisher: | Elsevier Science BV |
Issue Date: | 2004 |
ISSN: | 1570-0232 1873-376X |
Abstract: | A method for the quantitative determination of perhexiline and its main hydroxylated metabolites in human plasma, based on liquid chromatography-mass spectrometry (LC-MS), was developed. The method used protein precipitation with acetonitrile followed by dilution with water and subsequent direct injection of the extract into the LC-MS system. Hexadiline was used as internal standard and the intra-assay coefficients of variation were <or=5% for perhexiline and cis-hydroxyperhexiline over the target concentration range in patients. The lower limits of quantification were 0.005mg/l for perhexiline and 0.015mg/l for cis-hydroxyperhexiline, and the measuring ranges were from 0.05 to 3.0 and from 0.2 to 6.0mg/l, respectively. The method was compared with an established HPLC method with fluorescence detection and the correlation between the methods was close to 1 for both compounds. The predominant form of hydroxyperhexiline in 87% of the patient samples was found to be one of the diastereomeric pairs of cis-hydroxyperhexiline. In patients not forming this metabolite, trans-hydroxyperhexiline could be detected. We conclude that the present LC-MS method is suitable for use in a clinical routine laboratory. |
Keywords: | Humans Perhexiline Cardiovascular Agents Chromatography, High Pressure Liquid Isomerism Reference Standards Mass Spectrometry |
DOI: | 10.1016/j.jchromb.2004.02.019 |
Published version: | http://dx.doi.org/10.1016/j.jchromb.2004.02.019 |
Appears in Collections: | Aurora harvest 7 Pharmacology publications |
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