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https://hdl.handle.net/2440/1588
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Type: | Journal article |
Title: | Cytokine regulation of syndecan-1 and -2 gene expression in human periodontal fibroblasts and osteoblasts |
Author: | Worapamorn, W. Tam, S. Li, H. Haase, H. Bartold, P. |
Citation: | Journal of Periodontal Research, 2002; 37(4):273-278 |
Publisher: | Munksgaard Int Publ Ltd |
Issue Date: | 2002 |
ISSN: | 0022-3484 1600-0765 |
Statement of Responsibility: | Worapamorn W; Tam SP; Li H; Haase HR; Bartold PM |
Abstract: | Cell-surface proteoglycans participate in several biological functions including interactions with a variety of growth factors and cytokines. Regulation of syndecan-1 and -2 gene expression was investigated in human periodontal ligament fibroblasts (PDLF), osteoblasts (OB) and gingival fibroblasts (GF), in response to platelet-derived growth factor (PDGF-BB), transforming growth factor (TGF-beta 1), and interleukin (IL-1 beta) by Northern blot analyses. We also compared the effect of PDGF-BB and TGF-beta 1, separately and in combination, in the prolonged presence of IL-1 beta on the expression of both syndecan genes. The results demonstrated that the three cell lines regulated the expression of syndecan-1 and -2 in response to growth factors and cytokines in different manners. These cell lines increased syndecan-1 mRNA levels in response to either PDGF-BB or TGF-beta 1 and decreased levels in response to IL-1 beta. The effect of IL-1 beta on syndecan-1 mRNA synthesis was partially reversed after adding PDGF-BB and TGF-beta 1, separately or in combination, in the presence of IL-1 beta. In contrast, syndecan-2 mRNA level was markedly upregulated in response to either TGF-beta 1 or IL-1 beta in OB when compared with the other two cell lines. However, the stimulatory effect of TGF-beta 1 on syndecan-2 mRNA production in OB was abolished in the prolonged presence of IL-1 beta. These findings lend support to the notion that syndecan-1 and syndecan-2 have distinct functions which correlate with their source and functions within the periodontium. |
Keywords: | Alveolar Process Fibroblasts Osteoblasts Gingiva Periodontal Ligament Humans Proteoglycans Platelet-Derived Growth Factor Proto-Oncogene Proteins c-sis Transforming Growth Factor beta Membrane Glycoproteins RNA, Messenger Interleukin-1 Cytokines Blotting, Northern Cell Culture Techniques Gene Expression Regulation Up-Regulation Transforming Growth Factor beta1 Syndecan-1 Syndecans Syndecan-2 Statistics as Topic Becaplermin |
Description: | Copyright © 2002 Blackwell Munksgaard The definitive version is available at www.blackwell-synergy.com |
DOI: | 10.1034/j.1600-0765.2002.01610 |
Published version: | http://dx.doi.org/10.1034/j.1600-0765.2002.01610.x |
Appears in Collections: | Aurora harvest 6 Dentistry publications |
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