Identification of wheat-barley 2H alien substitution lines

Abstract

The genetic constitution of 15 materials derived from the cross wheat (cv. Chinese Spring) × barley (cv. Betzes) was analysed, and 6 disomic alien substitution lines were screened by genomic in situ hybridization. The chromosome configurations in pollen mother cells at meiotic metaphase I of F₁ from each disomic substitution line crossed with double ditelocentric lines 2A, 2B and 2D of Chinese Spring were observed, and a set of wheat-barley disomic alien substitution lines 2H(A), 2H(B) and 2H(D) were obtained. Restriction fragment length polymorphism analysis with the probe psr131 on the short arm of wheat homeologous group 2 combined with 4 restriction enzymes was carried out. The probe psr131 could be used as a molecular marker to tag the barley chromosomes 2B and 2D in vitality and other agronomic characters. The result of seed testing showed that the wheat starch appearance quality was changed from half-farinaceous (Chinese Spring) to half-cutin (substitution lines) by transferring the barley chromosome 2H to wheat.