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https://hdl.handle.net/2440/179
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Type: | Journal article |
Title: | Comparison of ELISA and RT-PCR for the detection of Prunus necrotic ring spot virus and prune dwarf virus in almond (Prunus dulcis) |
Author: | Mekuria, G. Ramesh, S. Alberts, E. Bertozzi, T. Wirthensohn, M. Collins, G. Sedgley, M. |
Citation: | Journal of Virological Methods, 2003; 114(1):65-69 |
Publisher: | Elsevier Science BV |
Issue Date: | 2003 |
ISSN: | 0166-0934 1879-0984 |
Statement of Responsibility: | Genet Mekuria, Sunita A. Ramesh, Evita Alberts, Terry Bertozzi, Michelle Wirthensohn, Graham Collins and Margaret Sedgley |
Abstract: | A technique based on the reverse transcriptase-polymerase chain reaction (RT-PCR) has been developed to detect the presence of Prunus necrotic ringspot virus (PNRSV) and prune dwarf virus (PDV) simultaneously in almond. This paper presents the results of a 3-year study comparing both enzyme-linked immunosorbent assay (ELISA) and RT-PCR for the detection of PNRSV and PDV using 175 almond leaf samples. Multiplex RT-PCR was found to be more sensitive than ELISA, especially when followed by nested PCR for the detection of PDV. The RT-PCR technique has the added advantage that plant material can be tested at any time throughout the growing season. |
Keywords: | Ilarvirus Prune dwarf virus ELISA Prunus necrotic ringspot virus |
Rights: | Copyright © 2003 Elsevier B.V. All rights reserved. |
DOI: | 10.1016/j.jviromet.2003.08.014 |
Description (link): | http://www.elsevier.com/wps/find/journaldescription.cws_home/506080/description#description |
Published version: | http://dx.doi.org/10.1016/j.jviromet.2003.08.014 |
Appears in Collections: | Agriculture, Food and Wine publications Aurora harvest 2 Environment Institute publications |
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