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https://hdl.handle.net/2440/23047
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dc.contributor.author | Cheney, K. | - |
dc.contributor.author | Kumar, R. | - |
dc.contributor.author | Purins, A. | - |
dc.contributor.author | Mundy, L. | - |
dc.contributor.author | Fergusson, A. | - |
dc.contributor.author | Shaw, D. | - |
dc.contributor.author | Burrell, C. | - |
dc.contributor.author | Li, P. | - |
dc.date.issued | 2006 | - |
dc.identifier.citation | AIDS Research and Human Retroviruses, 2006; 22(1):66-75 | - |
dc.identifier.issn | 0889-2229 | - |
dc.identifier.issn | 1931-8405 | - |
dc.identifier.uri | http://hdl.handle.net/2440/23047 | - |
dc.description.abstract | The establishment of reservoirs of latently infected cells is thought to contribute to the persistence of HIV-1 infection in the host. Studies so far have mainly focused on the long-lived reservoir of HIV-infected resting CD4+ T cells. A discrete population of HIV-infected CD4¯/CD8¯ double negative (DN) T cells has recently been shown to exist and may also play a role in HIV-1 persistence. DN T cells are CD3 positive, either TCRαβ or TCRγδ positive, but lack both CD4 and CD8 surface markers. We developed a novel, magnetic bead column-based cell fractionation procedure for isolating >99% pure DN T cells. CD4+, CD8+, and DN T cells were purified from 23 samples of a cohort of 18 HIV-1-infected patients. Each cell fraction was analyzed for levels of total and integrated HIV-1 DNA. A correlation was observed between the presence of HIV-1 DNA in the DN T cell fraction and plasma viral load (VL). Using a micrococulture technique, we saw an initial release of virus from DN T cells of a patient with high VL. Analysis of env and nef sequence data suggested that the HIV-1 present in CD4+ and DN T cells originated from a common infecting strain. Different from the published literature, we have demonstrated the presence of HIV-1 DNA in DN T cells only in patients who are experiencing HAART failure. While these cells may have a limited role in viral persistence in high VL patients, our results suggest DN T cells are unlikely to be a major reservoir in patients on HAART with clinically undetectable plasma viral RNA. | - |
dc.description.statementofresponsibility | Kelly M. Cheney, Raman Kumar, Adrian Purins, Linda Mundy, Wendy Ferguson, David Shaw, Christopher J. Burrell, and Peng LI. | - |
dc.language.iso | en | - |
dc.publisher | Mary Ann Liebert Inc Publ | - |
dc.rights | © Mary Ann Liebert, Inc | - |
dc.source.uri | http://www.liebertonline.com/doi/abs/10.1089/aid.2006.22.66 | - |
dc.subject | T-Lymphocytes | - |
dc.subject | Humans | - |
dc.subject | HIV-1 | - |
dc.subject | HIV Infections | - |
dc.subject | RNA, Viral | - |
dc.subject | Anti-HIV Agents | - |
dc.subject | Antiretroviral Therapy, Highly Active | - |
dc.subject | Viral Load | - |
dc.subject | Adult | - |
dc.subject | Middle Aged | - |
dc.subject | Male | - |
dc.subject | CD4 Antigens | - |
dc.subject | CD8 Antigens | - |
dc.title | HIV type 1 persistence in CD4¯/CD8¯ double negative T cells from patients on antiretroviral therapy | - |
dc.title.alternative | HIV type 1 persistence in CD4-bar/CD8-bar double negative T cells from patients on antiretroviral therapy | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1089/aid.2006.22.66 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Burrell, C. [0000-0002-4020-349X] | - |
Appears in Collections: | Aurora harvest 2 Public Health publications |
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