Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/23542
Citations | ||
Scopus | Web of ScienceĀ® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | Host-defence skin peptides of the Australian Streambank Froglet Crinia riparia: isolation and sequence determination by positive and negative ion electrospray mass spectrometry |
Author: | Maselli, V. Bilusich, D. Bowie, J. Tyler, M. |
Citation: | Rapid Communications in Mass Spectrometry, 2006; 20(5):797-803 |
Publisher: | John Wiley & Sons Ltd |
Issue Date: | 2006 |
ISSN: | 0951-4198 1097-0231 |
Statement of Responsibility: | Vita M. Maselli, Daniel Bilusich, John H. Bowie, Michael J. Tyler |
Abstract: | A combination of positive and negative ion electrospray mass spectrometry (ES-MS) together with automated Edman sequencing has been used to determine the amino acid sequences of the host-defence peptides from the skin glands of the froglet Crinia riparia. The peptides are called riparins. Of the eight peptides isolated, five are neuropeptides containing intramolecular disulfide linkages; e.g. the major peptide riparin 1.4 (FFLPPCAYKGTC-OH). Positive ion ES-MS identifies the five residues of riparin 1.4 outside the disulfide moiety, but provides no information on the sequence within the disulfide ring. In contrast, the negative ion dissociations of the [M-H]- ion of riparin 1.4 identify the --S-S-- link by loss of H2S2 from the [M-H]- ion, and also provide the sequence within the disulfide unit. Other peptides are riparin 2.1 [(IIEKLVNTALGLLSGL-NH2), a narrow-spectrum antibiotic], signiferin 3.1 [(GIAEFLNYIKSKA-NH2), an nNOS inhibitor] and riparin 5.1 [IVSYPDDAGEHAHKMG-NH2], which shows no neuropeptide, antibiotic or nNOS activity. |
Keywords: | Skin Animals Ranidae Neuropeptides Chromatography, High Pressure Liquid Spectrometry, Mass, Electrospray Ionization Sequence Analysis, Protein Amino Acid Sequence Molecular Sequence Data |
Description: | The definitive version may be found at www.wiley.com |
DOI: | 10.1002/rcm.2360 |
Published version: | http://dx.doi.org/10.1002/rcm.2360 |
Appears in Collections: | Aurora harvest 2 Chemistry publications |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.