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https://hdl.handle.net/2440/239
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Type: | Journal article |
Title: | Differential metal selectivity and gene expression of two zinc transporters from rice |
Author: | Ramesh, S. Shin, R. Eide, D. Schachtman, D. |
Citation: | Plant Physiology, 2003; 133(1):126-134 |
Publisher: | Amer Soc Plant Physiologists |
Issue Date: | 2003 |
ISSN: | 0032-0889 1532-2548 |
Abstract: | Zinc is an essential mineral for a wide variety of physiological and biochemical processes. To understand zinc transport in cereals, we identified putative zinc transporters in gene databases. Three full-length cDNAs were identified and characterized from rice (Oryza sativa). Two of the cDNAs partially complemented a yeast (Saccharomyces cerevisiae) mutant deficient in zinc uptake at low concentrations. The two transporters showed many similarities in function but differed in ionic selectivity and pH optimum of activity. Expression patterns also differed between the two genes. One gene was broadly expressed under all conditions, and the other gene was mainly induced by zinc deficiency to higher levels in roots than in leaves. Although the timing of expression differed between the two genes, localization of expression overlapped in roots. Comparisons of the protein sequences, ionic selectivity, and gene expression patterns of the two transporters suggest that they may play different roles in the physiology of the whole plant. |
Keywords: | Saccharomyces cerevisiae Zinc Carrier Proteins Membrane Transport Proteins Plant Proteins DNA, Complementary Cloning, Molecular Genetic Complementation Test Sequence Analysis, DNA Phylogeny Gene Expression Regulation, Plant Amino Acid Sequence Mutation Molecular Sequence Data Oryza |
DOI: | 10.1104/pp.103.026815 |
Published version: | http://dx.doi.org/10.1104/pp.103.026815 |
Appears in Collections: | Agriculture, Food and Wine publications Aurora harvest 6 |
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