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https://hdl.handle.net/2440/24036
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Type: | Journal article |
Title: | Recruitment and proliferation of CD4(+) T cells in synovium following adoptive transfer of adjuvant-induced arthritis |
Author: | Spargo, L. Cleland, L. Cockshell, M. Mayrhofer, G. |
Citation: | International Immunology, 2006; 18(6):897-910 |
Publisher: | Oxford Univ Press |
Issue Date: | 2006 |
ISSN: | 0953-8178 1460-2377 |
Statement of Responsibility: | Llewellyn D. J. Spargo, Leslie G. Cleland, Michaelia P. Cockshell and Graham Mayrhofer |
Abstract: | Adjuvant-induced arthritis can be transferred to naive Dark Agouti (DA) strain (DA.CD45.1) rats by thoracic duct (TD) lymphocytes. Disease can be re-induced in convalescent rats by further transfer of arthritogenic cells, suggesting that resolution of the adoptive disease is not due to active regulation. To examine whether resolution is due to exhaustion of effector cells, we transferred the disease to DA.CD45.1 recipients, using CD4+ T cells from DA.CD45.2 donors. At the height of the adoptively transferred disease, donor cells comprised only 5–10% of recirculating CD4+ T cells but they accounted for ~40% of the CD4+ T cells in synovium-rich tissues of the hind paws. Approximately 65% of the donor cells in the synovium expressed a marker of proliferation (Ki-67 antigen). Division of CD4+ T cells continued in shielded paws after suppression of the recirculating pool of lymphocytes by selective irradiation. Intravenously injected CD4+ TD T lymphoblasts from arthritic donors were recruited to normal paws and, in greater numbers, to paws of animals with existing arthritis. Survival of the [125I]iodo-deoxyuridine-labeled lymphoblasts was greater in animals with existing arthritis. We conclude that effector CD4+ T cells in target tissues can proliferate in response to autoantigens and exhibit enhanced survival. However, without a continuous supply, adoptively transferred effector cells do not produce autonomous local disease, due to limits to their lifespan and ability to replicate indefinitely. |
Keywords: | cell trafficking inflammation polyarthritis rat model T lymphocytes |
DOI: | 10.1093/intimm/dxl026 |
Published version: | http://intimm.oxfordjournals.org/cgi/content/abstract/18/6/897 |
Appears in Collections: | Aurora harvest 2 Molecular and Biomedical Science publications |
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