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https://hdl.handle.net/2440/27536
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Type: | Journal article |
Title: | MHC II+ CD45+ cells from synovium-rich tissues of normal rats: phenotype, comparison with macrophage and dendritic cell lineages and differentiation into mature dendritic cells in vitro |
Author: | Moghaddami, M. Cleland, L. Mayrhofer, G. |
Citation: | International Immunology, 2005; 17(8):1103-1115 |
Publisher: | Oxford Univ Press |
Issue Date: | 2005 |
ISSN: | 0953-8178 1460-2377 |
Statement of Responsibility: | Mahin Moghaddami, Leslie G. Cleland and Graham Mayrhofer |
Abstract: | Synovial tissues are frequent sites of inflammatory disorders in which dendritic cells (DCs) may play an important role. This study examines potential antigen-presenting cells obtained from synovium-rich tissues (SRTs) by vascular perfusion of rat hind limbs with collagenase and further enzymatic digestion of the disarticulated hind paws in vitro. The three sub-populations of interest were: CD45+MHC IIhi, mainly CD11c+ and CD163¯; CD45+MHC II¹°, mainly CD11c¯ and CD163+ and CD45+MHC II¯, mainly CD11c¯ and CD163+. Expression of CD11c and CD163 correlated with ruffled cell-surface (CD11c+CD163¯) and highly vacuolated cytoplasm (CD11c¯CD163+), respectively. Culture of the CD45+CD163¯ sub-population in granulocyte macrophage colony-stimulating factor (GM-CSF) yielded CD45+MHC IIhi CD11c+CD163¯ cells with veiled morphology, while the large vacuolated cells that expressed CD163 resembled type A synoviocytes in both surface antigen phenotype and morphology. These results demonstrate that SRTs contain indeterminate cells that can differentiate into mature DCs in vitro in response to GM-CSF, plus mature synovial lining macrophages. |
Keywords: | animal model antigen-presenting cells cytokines polyarthritis synovial cells |
Description: | Copyright © 2005 Japanese Society for Immunology |
DOI: | 10.1093/intimm/dxh290 |
Published version: | http://intimm.oxfordjournals.org/cgi/content/abstract/17/8/1103 |
Appears in Collections: | Aurora harvest 2 Molecular and Biomedical Science publications |
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