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https://hdl.handle.net/2440/27602
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Type: | Journal article |
Title: | Phosphorylation inhibits DNA-binding of alternatively spliced aryl hydrocarbon receptor nuclear translocator |
Author: | Kewley, R. Whitelaw, M. |
Citation: | Biochemical and Biophysical Research Communications, 2005; 338(1):660-667 |
Publisher: | Academic Press Inc |
Issue Date: | 2005 |
ISSN: | 0006-291X 1090-2104 |
Organisation: | Centre for the Molecular Genetics of Development |
Statement of Responsibility: | Robyn J. Kewley, and Murray L. Whitelaw |
Abstract: | The basic helix–loop–helix/PER-ARNT-SIM homology (bHLH/PAS) transcription factor ARNT (aryl hydrocarbon receptor nuclear translocator) is a key component of various pathways which induce the transcription of cytochrome P450 and hypoxia response genes. ARNT can be alternatively spliced to express Alt ARNT, containing an additional 15 amino acids immediately N-terminal to the DNA-binding basic region. Here, we show that ARNT and Alt ARNT proteins are differentially phosphorylated by protein kinase CKII in vitro. Phosphorylation had an inhibitory effect on DNA-binding to an E-box probe by Alt ARNT, but not ARNT, homodimers. This inhibitory phosphorylation occurs through Ser77. Moreover, a point mutant, Alt ARNT S77A, shows increased activity on an E-box reporter gene, consistent with Ser77 being a regulatory site in vivo. In contrast, DNA binding by an Alt ARNT/dioxin receptor heterodimer to the xenobiotic response element is not inhibited by phosphorylation with CKII, nor does Alt ARNT S77A behave differently from wild type Alt ARNT in the context of a dioxin receptor heterodimer. |
Keywords: | Transcription bHLH/PAS proteins ARNT Dioxin receptor |
Description: | Copyright © 2005 Elsevier Inc. All rights reserved. |
DOI: | 10.1016/j.bbrc.2005.08.073 |
Description (link): | http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/description#description |
Published version: | http://dx.doi.org/10.1016/j.bbrc.2005.08.073 |
Appears in Collections: | Aurora harvest 6 Centre for the Molecular Genetics of Development publications Molecular and Biomedical Science publications |
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