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https://hdl.handle.net/2440/27915
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Type: | Journal article |
Title: | DNA fingerprinting probe specific to isolates of Rhizoctonia solani AG-3 |
Author: | Balali, G. Whisson, D. Scott, E. Neate, S. |
Citation: | Fungal Biology, 1996; 100(4):467-470 |
Publisher: | CAMBRIDGE UNIV PRESS |
Issue Date: | 1996 |
ISSN: | 0953-7562 1469-8102 |
Abstract: | Anastomosis group 3 (AG-3) isolates of Rhizoctonia solani that cause black scurf were recovered from diseased potatoes in Virginia and Lenswood in South Australia. A highly repeated and specific DNA fingerprinting probe, pR3718, prepared from AG-3 isolate R37, hybridized to Southem blots of all AG-3 isolates tested, but had no or very weak reactions with isolates from other anastomosis groups. Genetic variation among the isolates was observed using the fingerprinting probe. Sac I or Hind III restriction endonuclease digests of DNA prepared from 136 AG-3 isolates revealed 38 fingerprint patterns, each containing 12-19 Sac I or Hind III fragments. Because the pR3718 clone recognizes a DNA sequence with a high number of copies per genome and appears to be specific for AG-3 isolates of Rhizoctonia, it has the potential to be used as a diagnostic tool to study the epidemiology, genetic diversity and population dynamics of this important plant pathogen. |
DOI: | 10.1016/S0953-7562(96)80145-6 |
Published version: | http://dx.doi.org/10.1016/s0953-7562(96)80145-6 |
Appears in Collections: | Agriculture, Food and Wine publications Aurora harvest 2 |
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