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https://hdl.handle.net/2440/28046
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Type: | Journal article |
Title: | Nucleotide sequence of the mouse VEGF 3'UTR and quantitative analysis of sites of polyadenylation |
Author: | Dibbens, J. Polyak, S. Damert, A. Risau, W. Vadas, M. Goodall, G. |
Citation: | Biochimica et Biophysica Acta: Gene Regulatory Mechanisms, 2001; 1518(01-Feb):57-62 |
Publisher: | Elsevier Science BV |
Issue Date: | 2001 |
ISSN: | 0167-4781 1878-2434 |
Statement of Responsibility: | J. A. Dibbens, S. W. Polyak, A. Damert, W. Risau, M. A. Vadas and G. J. Goodall |
Abstract: | Sequencing of rat and human vascular endothelial growth factor (VEGF) cDNA clones has previously identified a 3' untranslated region of approximately 1.9 kb, although the apparent site of polyadenylation differed in the two species, despite a high degree of sequence conservation in the region. Neither site is preceded by a canonical AAUAAA polyadenylation signal, a situation frequently found in genes that are subject to alternative polyadenylation. We have sequenced 2.25 kb of the 3' region of the mouse VEGF gene and mapped the usage of potential polyadenylation sites in fibroblasts cultured under both normoxic and hypoxic conditions. We find that two sites for polyadenylation are present in the mouse VEGF gene but the majority of transcripts contain the longer form of the 3'UTR and that their usage is not effected by environmental oxygen tension. |
Keywords: | 3T3 Cells Animals Humans Mice Rats Nucleotides Vascular Endothelial Growth Factors Vascular Endothelial Growth Factor A Endothelial Growth Factors 3' Untranslated Regions Poly A Lymphokines Sequence Analysis, RNA Binding Sites Base Sequence Molecular Sequence Data |
Description: | Copyright © 2001 Elsevier Science B.V. All rights reserved. |
DOI: | 10.1016/S0167-4781(01)00167-1 |
Description (link): | http://www.elsevier.com/wps/find/journaldescription.cws_home/506065/description#description |
Published version: | http://dx.doi.org/10.1016/s0167-4781(01)00167-1 |
Appears in Collections: | Aurora harvest 2 Molecular and Biomedical Science publications |
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