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https://hdl.handle.net/2440/28082
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Type: | Journal article |
Title: | A simple method for the assay of colistin in human plasma, using pre-column derivatization with 9-fluorenylmethyl chloroformate in solid-phase extraction cartridges and reversed-phase high-performance liquid chromatography |
Author: | Li, J. Milne, R. Nation, R. Turnidge, J. Coulthard, K. Johnson, D. |
Citation: | Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 2001; 761(2):167-175 |
Publisher: | Elsevier Science BV |
Issue Date: | 2001 |
ISSN: | 1570-0232 0378-4347 |
Abstract: | A simple, selective and sensitive high-performance liquid chromatographic (HPLC) method is described for the determination of colistin in human plasma. Derivatization with 9-fluorenylmethyl chloroformate was performed in the same solid-phase extraction C18 cartridge used for sample pre-treatment, followed by reversed-phase HPLC with fluorimetric detection. Quantification was achieved using the ratio of the summed peak areas of colistin A and B derivatives to that of the derivative of netilmicin (internal standard). Linear calibration curves were obtained within the concentrations of colistin sulfate from 0.10 to 4.0 mg/l in plasma. Accuracy was within 10% and reproducibility (RSD) was less than 10%. |
Keywords: | Humans Colistin Fluorenes Fluorescent Dyes Calibration Chromatography, High Pressure Liquid Sensitivity and Specificity Reproducibility of Results Mass Spectrometry |
DOI: | 10.1016/S0378-4347(01)00326-7 |
Published version: | http://dx.doi.org/10.1016/s0378-4347(01)00326-7 |
Appears in Collections: | Aurora harvest 6 Molecular and Biomedical Science publications |
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