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https://hdl.handle.net/2440/28195
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Type: | Journal article |
Title: | The WD40-repeat protein CreC interacts with and stabilizes the deubiquitinating enzyme CreB in vivo in Aspergillus nidulans |
Author: | Lockington, R. Kelly, J. |
Citation: | Molecular Microbiology, 2002; 43(5):1173-1182 |
Publisher: | Blackwell Science Ltd |
Issue Date: | 2002 |
ISSN: | 0950-382X 1365-2958 |
Statement of Responsibility: | Robin A. Lockington and Joan M. Kelly |
Abstract: | Genetic dissection of carbon catabolite repression in Aspergillus nidulans has identified two genes, creB and creC, which, when mutated, affect expression of many genes in both carbon catabolite repressing and derepressing conditions. The creB gene encodes a functional deubiquitinating enzyme and the creC gene encodes a protein that contains five WD40 repeat motifs, and a proline-rich region . These findings have allowed the in vivo molecular analysis of a cellular switch involving deubiquitination. We demonstrate that overexpression of the CreB deubiquitinating enzyme can partially compensate for a lack of the CreC WD40-repeat protein in the cell, but not vice versa and, thus, the CreB deubiquitinating enzyme acts downstream of the CreC WD40-repeat protein. We demonstrate using co-immunoprecipitation experiments that the CreB deubiquitinating enzyme and the CreC WD40-repeat protein interact in vivo in both carbon catabolite repressing and carbon catabolite derepressing conditions. Further, we show that the CreC WD40-repeat protein is required to prevent the proteolysis of the CreB deubiquitinating enzyme in the absence of carbon catabolite repression. This is the first case in which a regulatory deubiquitinating enzyme has been shown to interact with another protein that is required for the stability of the deubiquitinating enzyme. |
Keywords: | Aspergillus nidulans Endopeptidases Protein Kinases Fungal Proteins Ubiquitins Precipitin Tests Repetitive Sequences, Amino Acid Mutation |
Description: | The definitive version is available at www.blackwell-synergy.com |
DOI: | 10.1046/j.1365-2958.2002.02811.x |
Published version: | http://dx.doi.org/10.1046/j.1365-2958.2002.02811.x |
Appears in Collections: | Aurora harvest 2 Molecular and Biomedical Science publications |
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