Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/2998
Citations | ||
Scopus | Web of ScienceĀ® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | Lipopolysaccharide O antigen chains mask IcsA (VirG) in Shigella flexneri |
Author: | Morona, R. Van Den Bosch, L. |
Citation: | FEMS Microbiology Letters, 2003; 221(2):173-180 |
Publisher: | Elsevier Science BV |
Issue Date: | 2003 |
ISSN: | 0378-1097 1574-6968 |
Statement of Responsibility: | Renato Morona, and Luisa Van Den Bosch |
Abstract: | Shigella flexneri 2a strain 2457T lipopolysaccharide (LPS) has O antigen (Oag) chains with two modal lengths (S-type and VL-type), and has IcsA apparently located at one pole on its cell surface. Treatment of Y serotype derivatives of 2457T and RMA696 (2457T wzzSF) with Sf6 tailspike protein (TSP) resulted in hydrolysis of Oag chains, and an increase in detection of IcsA by indirect immunofluorescence staining on both the lateral and polar regions of the cell surface. Newly synthesised IcsA expressed from a pBAD promoter in a S. flexneri Y strain was also detected on both the lateral and polar regions of the cell when incubated with TSP prior to immunofluorescence staining. We conclude that IcsA is actually located on both lateral and polar regions of the S. flexneri cell surface, and that LPS Oag chains mask the presence of IcsA by hindering its detection with antibodies. These results have implications for the mechanism of IcsA export. They suggest that while IcsA export is predominantly targeted to the old cell pole, it can also occur on the lateral regions of the cell surface. |
Keywords: | Shigella flexneri Lipopolysaccharides O Antigens Bacterial Proteins DNA-Binding Proteins Transcription Factors Fluorescent Antibody Technique Plasmids |
Description: | The definitive version is available at www.blackwell-synergy.com |
DOI: | 10.1016/S0378-1097(03)00210-6 |
Published version: | http://dx.doi.org/10.1016/s0378-1097(03)00210-6 |
Appears in Collections: | Aurora harvest 6 Molecular and Biomedical Science publications |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.