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https://hdl.handle.net/2440/2999
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Type: | Journal article |
Title: | Multicopy icsA is able to suppress the virulence defect caused by wzzSF mutation in the Shigella flexneri |
Author: | Morona, R. Van Den Bosch, L. |
Citation: | FEMS Microbiology Letters, 2003; 221(2):213-219 |
Publisher: | Elsevier Science BV |
Issue Date: | 2003 |
ISSN: | 0378-1097 1574-6968 |
Statement of Responsibility: | Renato Morona and Luisa Van Den Bosch |
Abstract: | The lipopolysaccharides (LPS) of Shigella flexneri are important for virulence and their O antigen (Oag) polysaccharide chains affect IcsA (VirG)-mediated actin-based motility (ABM) within mammalian cells. S. flexneri 2a 2457T has smooth LPS whose Oag chains have two modal lengths (short (S)-type and very long (VL)-type), and has IcsA predominantly located at one pole on its cell surface. A S. flexneri 2457T wzzSF mutant (RMA696) has VL-type Oag but not S-type Oag chains, less IcsA detectable by immunofluorescence on its cell surface, reduced virulence and defective ABM. Introduction of a plasmid encoding IcsA into S. flexneri wzzSF showed that multicopy icsA could suppress the virulence defects (Sereny reaction, HeLa cell monolayer plaquing, and F-actin comet tail formation) caused by the wzzSF mutation suggesting that the VL-type Oag chains were masking IcsA and limiting the amount available to initiate ABM. |
Keywords: | Hela Cells Humans Shigella flexneri Lipopolysaccharides O Antigens Bacterial Proteins DNA-Binding Proteins Transcription Factors Fluorescent Antibody Technique Virulence Mutation |
Description: | The definitive version is available at www.blackwell-synergy.com |
DOI: | 10.1016/S0378-1097(03)00217-9 |
Published version: | http://dx.doi.org/10.1016/s0378-1097(03)00217-9 |
Appears in Collections: | Aurora harvest 6 Molecular and Biomedical Science publications |
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