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https://hdl.handle.net/2440/34794
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Type: | Journal article |
Title: | Inhibition of generation of cytotoxic T lymphocyte activity by a CCL19/macrophage inflammatory protein (MIP)-3beta antagonist |
Author: | Pilkington, K. Clark-Lewis, I. McColl, S. |
Citation: | Journal of Biological Chemistry, 2004; 279(39):40276-40282 |
Publisher: | Amer Soc Biochemistry Molecular Biology Inc |
Issue Date: | 2004 |
ISSN: | 0021-9258 1083-351X |
Statement of Responsibility: | Katherine R. Pilkington, Ian Clark-Lewis, and Shaun R. McColl |
Abstract: | Chemokines constitute a group of over 40 secreted peptides that are important for the control of leukocyte migration both during homeostasis and inflammation. Recent studies have implicated the ligands CCL19 and CCL21 and their receptor, CCR7, in the specific migration of naïve lymphocytes and mature dendritic cells to secondary lymphoid organs during immune homeostasis. However, the role that these molecules play during immune priming is not well understood. In this study, using CCL19(8–83), a novel N-terminal truncation mutant, we have investigated the role of CCL19 in a primary allogeneic immune response, a response of particular relevance to transplant rejection. This antagonist specifically inhibited wild type CCL19-induced chemotaxis and intracellular calcium mobilization without affecting that of CCL21. The treatment of mice with CCL19(8–83) did not globally inhibit the recruitment of cells into lymph nodes; however, it inhibited the generation of cytotoxic T lymphocytes toward allogeneic dendritic cells. This is the first evidence that CCL19 plays a role in immune priming. |
Keywords: | Spleen Dendritic Cells Leukocytes T-Lymphocytes, Cytotoxic Immune System Animals Mice, Inbred BALB C Humans Mice Inflammation Calcium Peptides Receptors, Chemokine Chemokines Chemokines, CC Ligands Cell Movement Chemotaxis Amino Acid Sequence Protein Structure, Tertiary Protein Binding Dose-Response Relationship, Drug Mutation Time Factors Molecular Sequence Data Female Receptors, CCR7 Chemokine CCL19 Chemokine CCL21 |
Description: | Copyright © 2004 by the American Society for Biochemistry and Molecular Biology. |
DOI: | 10.1074/jbc.M405808200 |
Published version: | http://www.jbc.org/cgi/content/abstract/279/39/40276 |
Appears in Collections: | Aurora harvest 6 Molecular and Biomedical Science publications |
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