Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/34821
Citations | ||
Scopus | Web of Science® | Altmetric |
---|---|---|
?
|
?
|
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Chapman, M. | - |
dc.contributor.author | Charchar, F. | - |
dc.contributor.author | Kinston, S. | - |
dc.contributor.author | Bird, C. | - |
dc.contributor.author | Grafham, D. | - |
dc.contributor.author | Rogers, J. | - |
dc.contributor.author | Grutzner, F. | - |
dc.contributor.author | Graves, J. | - |
dc.contributor.author | Green, A. | - |
dc.contributor.author | Gottgens, B. | - |
dc.date.issued | 2003 | - |
dc.identifier.citation | Genomics, 2003; 81(3):249-259 | - |
dc.identifier.issn | 0888-7543 | - |
dc.identifier.issn | 1089-8646 | - |
dc.identifier.uri | http://hdl.handle.net/2440/34821 | - |
dc.description | Copyright © 2003 Elsevier Science | - |
dc.description.abstract | Comparative genomic sequence analysis is a powerful technique for identifying regulatory regions in genomic DNA. However, its utility largely depends on the evolutionary distances between the species involved. Here we describe the screening of a genomic BAC library from the stripe-faced dunnart, Sminthopsis macroura, formerly known as the narrow-footed marsupial mouse. We isolated a clone containing the LYL1 locus, completely sequenced the 60.6-kb insert, and compared it with orthologous human and mouse sequences. Noncoding homology was substantially reduced in the human/dunnart analysis compared with human/mouse, yet we could readily identify all promoters and exons. Human/mouse/dunnart alignments of the LYL1 candidate promoter allowed us to identify putative transcription factor binding sites, revealing a pattern highly reminiscent of critical regulatory regions of the LYL1 paralogue, SCL. This newly identified LYL1 promoter showed strong activity in myeloid progenitor cells and was bound in vivo by Fli1, Elf1, and Gata2-transcription factors all previously shown to bind to the SCL stem cell enhancer. This study represents the first large-scale comparative analysis involving marsupial genomic sequence and demonstrates that such comparisons provide a powerful approach to characterizing mammalian regulatory elements. | - |
dc.description.statementofresponsibility | Michael A. Chapman, Fadi J. Charchar, Sarah Kinston, Christine P. Bird, Darren Grafham, Jane Rogers, Frank Grützner, Jennifer A. Marshall Graves, Anthony R. Green and Berthold Göttgens | - |
dc.language.iso | en | - |
dc.publisher | Academic Press Inc Elsevier Science | - |
dc.source.uri | http://www.elsevier.com/wps/locate/issn/08887543 | - |
dc.subject | Animals | - |
dc.subject | Marsupialia | - |
dc.subject | DNA-Binding Proteins | - |
dc.subject | Neoplasm Proteins | - |
dc.subject | DNA | - |
dc.subject | Chromosome Mapping | - |
dc.subject | Phylogeny | - |
dc.subject | Amino Acid Sequence | - |
dc.subject | Base Sequence | - |
dc.subject | Sequence Homology, Amino Acid | - |
dc.subject | Sequence Homology, Nucleic Acid | - |
dc.subject | Models, Genetic | - |
dc.subject | Molecular Sequence Data | - |
dc.subject | Basic Helix-Loop-Helix Transcription Factors | - |
dc.subject | Promoter Regions, Genetic | - |
dc.title | Comparative and functional analyses of LYL1 loci establish marsupial sequences as a model for phylogenetic footprinting | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1016/S0888-7543(03)00005-3 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Grutzner, F. [0000-0002-3088-7314] | - |
Appears in Collections: | Aurora harvest 6 Environment Institute Leaders publications Molecular and Biomedical Science publications |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.