Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/37066
Citations | ||
Scopus | Web of ScienceĀ® | Altmetric |
---|---|---|
?
|
?
|
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Coldwell, J. | - |
dc.contributor.author | Phillis, B. | - |
dc.contributor.author | Sutherland, K. | - |
dc.contributor.author | Howarth, G. | - |
dc.contributor.author | Blackshaw, L. | - |
dc.date.issued | 2007 | - |
dc.identifier.citation | The Journal of Physiology, 2007; 579(1):203-213 | - |
dc.identifier.issn | 0022-3751 | - |
dc.identifier.issn | 1469-7793 | - |
dc.identifier.uri | http://hdl.handle.net/2440/37066 | - |
dc.description.abstract | 5-Hydroxytryptamine (5-HT) activates colonic splanchnic afferents, a mechanism by which it has been implicated in generating symptoms in postinfectious and postinflammatory states in humans. Here we compared mechanisms of colonic afferent activation by 5-HT and mechanical stimuli in normal and inflamed rat colon, and after recovery from inflammation. Colonic inflammation was induced in rats by dextran sulphate sodium. Single-fibre recordings of colonic lumbar splanchnic afferents revealed that 58% of endings responded to 5-HT (10(-4) m) in controls, 88% in acute inflammation (P<0.05) and 75% after 21 days recovery (P < 0.05 versus control). Maximal responses to 5-HT were also larger, and the estimated EC50 was reduced from 3.2 x 10(-6) to 8 x 10(-7) m in acute inflammation and recovered to 2 x 10(-6) m after recovery. Responsiveness to mechanical stimulation was unaffected. 5-HT3 receptor antagonism with alosetron reduced responses to 5-HT in controls but not during inflammation. Responses to the mast cell degranulator 48/80 mimicked those to 5-HT in inflamed tissue but not in controls, and more 5-HT-containing mast cells were seen close to calcitonin gene-related peptide-containing fibres in inflamed serosa. We conclude that colonic serosal and mesenteric endings exhibit increased sensitivity to 5-HT in inflammation, with both an increase in proportion of responders and an increase in sensitivity, which is maintained after healing of inflammation. This is associated with alterations in the roles of 5-HT3 receptors and mast cells. | - |
dc.description.statementofresponsibility | Jonathan R. Coldwell, Benjamin D. Phillis, Kate Sutherland, Gordon S. Howarth, and L. Ashley Blackshaw | - |
dc.language.iso | en | - |
dc.publisher | Blackwell Publishing Ltd | - |
dc.source.uri | http://dx.doi.org/10.1113/jphysiol.2006.123158 | - |
dc.subject | Colon | - |
dc.subject | Afferent Pathways | - |
dc.subject | Nerve Fibers | - |
dc.subject | Splanchnic Nerves | - |
dc.subject | Mast Cells | - |
dc.subject | Animals | - |
dc.subject | Rats | - |
dc.subject | Rats, Sprague-Dawley | - |
dc.subject | Colitis | - |
dc.subject | Disease Models, Animal | - |
dc.subject | Serotonin | - |
dc.subject | Calcitonin Gene-Related Peptide | - |
dc.subject | Physical Stimulation | - |
dc.subject | Electrophysiology | - |
dc.subject | Recovery of Function | - |
dc.subject | Cell Degranulation | - |
dc.subject | Action Potentials | - |
dc.subject | Biomarkers | - |
dc.title | Increased responsiveness of rat colonic splanchnic afferents to 5-HT after inflammation and recovery | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1113/jphysiol.2006.123158 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Howarth, G. [0000-0001-6979-6084] | - |
dc.identifier.orcid | Blackshaw, L. [0000-0003-1565-0850] | - |
Appears in Collections: | Aurora harvest 6 Molecular and Biomedical Science publications |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.