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https://hdl.handle.net/2440/38890
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DC Field | Value | Language |
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dc.contributor.author | Johnson, D. | - |
dc.contributor.author | Polyak, S. | - |
dc.contributor.author | Wallace, J. | - |
dc.contributor.author | Martin, L. | - |
dc.date.issued | 2003 | - |
dc.identifier.citation | International Journal of Peptide Research and Therapeutics, 2003; 10(5-6):495-500 | - |
dc.identifier.issn | 1573-3149 | - |
dc.identifier.issn | 1573-3904 | - |
dc.identifier.uri | http://hdl.handle.net/2440/38890 | - |
dc.description | The original publication can be found at www.springerlink.com | - |
dc.description.abstract | Thioredoxin, a redox active disulfide protein, has been specifically immobilized at a modified gold electrode. The thioredoxin is uniquely oriented relative to the electrode surface via a histidine tag thereby enabling the redox mechanism of protein to be examined. When scanning the applied potential in the negative direction (cathodic), two one-electron reduction waves can be observed. The first of these redox waves occurs at −90 mV and is electrochemically reversible at all scan rates whereas the second wave occurs at −433 mV is irreversible. These two processes are interpreted as the initial reduction of the disulfide form of the protein to a stable (reversible) semi-reduced radical anion intermediate, followed by an electrochemically irreversible process to form a fully reduced thioredoxin. These electron transfer characteristics suggest that a radical intermediate retaining the sulfur-sulfur bond is thermodynamically stable but the addition of a second electron results in bond scission. | - |
dc.description.statementofresponsibility | Daniel L. Johnson, Steven W. Polyak, John C. Wallace and Lisandra L. Martin | - |
dc.language.iso | en | - |
dc.publisher | Springer Netherlands | - |
dc.source.uri | http://dx.doi.org/10.1007/bf02442581 | - |
dc.subject | direct electrochemistry | - |
dc.subject | disulfide protein | - |
dc.subject | gold electrode | - |
dc.subject | metal affinity | - |
dc.subject | protein immobilization | - |
dc.subject | radical intermediate | - |
dc.subject | thioredoxin | - |
dc.title | Probing the stability of the disulfide radical intermediate of thioredoxin using direct electrochemistry | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1007/BF02442581 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Polyak, S. [0000-0002-8458-5194] | - |
Appears in Collections: | Aurora harvest Molecular and Biomedical Science publications |
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