Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/40813
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Type: | Journal article |
Title: | Association of a missense mutation in the bovine leptin gene with carcass fat content and leptin mRNA levels |
Author: | Buchanan, Fiona C. Fitzsimmons, Carolyn Jean Van Kessel, Andrew G. Thue, Tracey D. Winkelman-Sim, Dianne C. Schmutz, Sheila M. |
Citation: | Selection Genetics Evolution, 2002; 34(1):105-116 |
Publisher: | EDP Sciences |
Issue Date: | 2002 |
School/Discipline: | School of Agriculture, Food and Wine : Agricultural and Animal Science |
Abstract: | Previously, we have shown that alleles of the BM1500 microsatellite, located 3.6 kb downstream of the leptin gene in cattle, were associated with carcass fat measures in a population of 154 unrelated beef bulls. Subsequently, a cytosine (C) to thymine (T) transition that encoded an amino acid change of an arginine to a cysteine was identified in exon 2 of the leptin gene. A PCR-RFLP was designed and allele frequencies in four beef breeds were correlated with levels of carcass fat. The T allele was associated with fatter carcasses and the C allele with leaner carcasses. The frequencies of the SNP alleles among breeds indicated that British breeds have a higher frequency of the T allele whereas the continental breeds have a higher occurrence of the C allele. A ribonuclease protection assay was developed to quantify leptin mRNA in a separate group of animals selected by genotype. Animals homozygous for thymine expressed higher levels of leptin mRNA. This may suggest that the T allele, which adds an extra cysteine to the protein, imparts a partial loss of biological function and hence could be the causative mutation. |
Keywords: | Leptin; cattle; obese; fat; marbling |
Rights: | © INRA, EDP Sciences 2002 |
DOI: | 10.1051/gse:2001006 |
Appears in Collections: | Agriculture, Food and Wine publications |
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