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https://hdl.handle.net/2440/42308
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Type: | Journal article |
Title: | Leukocyte Migration in Adipose Tissue of Mice Null for ICAM-1 and Mac-1 Adhesion Receptors |
Author: | Robker, R. Collins, R. Beaudet, A. Mersmann, H. Smith, C. |
Citation: | Obesity, 2004; 12(6):936-940 |
Publisher: | North Amer Assoc Study Obesity |
Issue Date: | 2004 |
ISSN: | 1930-7381 1550-8528 |
Statement of Responsibility: | Rebecca L. Robker, Robert G. Collins, Arthur L. Beaudet, Harry J. Mersmann and C. Wayne Smith |
Abstract: | Objective: To determine whether the leukocyte adhesion receptors ICAM-1 and Mac-1, regulators of immune cell migration, have an intrinsic role within adipose tissue by 1) analyzing the expression of ICAM-1 in adipose tissue, 2) identifying leukocyte populations within adipose tissue, and 3) determining whether ICAM-1 and Mac-1 mutant mice exhibit abnormal numbers of adipose tissue leukocytes. Research Methods and Procedures: Wild-type, ICAM-1-/-, and Mac-1-/- mice were fed a long-term high-fat diet. ICAM-1 expression was analyzed by Northern blot and immunohistochemistry. Leukocytes within adipose tissue were identified by immunohistochemistry and flow cytometry. Results: ICAM-1 was expressed in adipose tissue and localized to the vascular endothelium. Macrophages and lymphocytes were prevalent within the stromal-vascular cell fraction of adipose tissue, and gender-specific differences were observed, with adipose tissue from female mice containing significantly more macrophages than tissue from male mice. Numbers of leukocytes in ICAM-1-/- and Mac-1-/- mice were not different from wild-types, however, indicating that these adhesion receptors are not required for leukocyte migration into adipose tissue. Discussion: Our results documented leukocyte populations within adipose tissue, which may be involved in the development of heightened inflammation that is characteristic of obesity. |
Keywords: | Endothelium, Vascular Adipose Tissue Leukocytes Animals Mice, Knockout Mice Intercellular Adhesion Molecule-1 Macrophage-1 Antigen RNA Blotting, Northern Flow Cytometry Immunohistochemistry Cell Movement Gene Expression Regulation Female Male |
Rights: | © 2004 The Obesity Society |
DOI: | 10.1038/oby.2004.114 |
Published version: | http://dx.doi.org/10.1038/oby.2004.114 |
Appears in Collections: | Aurora harvest Obstetrics and Gynaecology publications |
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