Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/43543
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dc.contributor.author | Moghaddami, M. | - |
dc.contributor.author | Cleland, L. | - |
dc.contributor.author | Radisic, G. | - |
dc.contributor.author | Mayrhofer, G. | - |
dc.date.issued | 2007 | - |
dc.identifier.citation | Arthritis Research and Therapy, 2007; 9(6):1-13 | - |
dc.identifier.issn | 1478-6354 | - |
dc.identifier.issn | 1478-6362 | - |
dc.identifier.uri | http://hdl.handle.net/2440/43543 | - |
dc.description | This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. | - |
dc.description.abstract | Adoptive transfer of adjuvant-induced arthritis was used in this study to examine local macrophages and dendritic cells (DCs) during T cell-mediated synovial inflammation. We studied the influx of CD11b+CD11c+ putative myeloid DCs and other non-lymphoid CD45+ cells into synovium-rich tissues (SRTs) of the affected hind paws in response to a pulse of autoreactive thoracic duct cells. Cells were prepared from the SRTs using a collagenase perfusion-digestion technique, thus allowing enumeration and phenotypic analysis by flow cytometry. Numbers of CD45+ cells increased during the first 6 days, with increases in CD45+MHC (major histocompatibility complex) II+ monocyte-like cells from as early as day 3 after transfer. In contrast, typical MHC II- monocytes, mainly of the CD4- subset, did not increase until 12 to 14 days after cell transfer, coinciding with the main influx of polymorphonuclear cells. By day 14, CD45+MHC IIhi cells constituted approximately half of all CD45+ cells in SRT. Most of the MHC IIhi cells expressed CD11c and CD11b and represented putative myeloid DCs, whereas only approximately 20% were CD163+ macrophages. Less than 5% of the MHC IIhi cells in inflamed SRT were CD11b-, setting a maximum for any influx of plasmacytoid DCs. Of the putative myeloid DCs, a third expressed CD4 and both the CD4+ and the CD4- subsets expressed the co-stimulatory molecule CD172a. Early accumulation of MHC IIhiCD11c+ monocyte-like cells during the early phase of T cell-mediated inflammation, relative to typical MHC II- blood monocytes, suggests that recruited monocytes differentiate rapidly toward the DC lineage at this stage in the disease process. However, it is possible also that the MHC IIhiCD11c+ cells originate from a specific subset of DC-like circulating mononuclear cells. | - |
dc.description.statementofresponsibility | Mahin Moghaddami, Leslie G Cleland, Gorjana Radisic and Graham Mayrhofer | - |
dc.language.iso | en | - |
dc.publisher | BioMed Central Ltd. | - |
dc.rights | Copyright © 2007 Moghaddami et al.; licensee BioMed Central Ltd. | - |
dc.source.uri | http://arthritis-research.com/content/9/6/R120 | - |
dc.subject | Dendritic Cells | - |
dc.subject | T-Lymphocytes | - |
dc.subject | Macrophages | - |
dc.subject | Animals | - |
dc.subject | Rats, Inbred Strains | - |
dc.subject | Rats | - |
dc.subject | Arthritis, Experimental | - |
dc.subject | Synovitis | - |
dc.subject | Intercellular Adhesion Molecule-1 | - |
dc.subject | Adoptive Transfer | - |
dc.subject | Cell Movement | - |
dc.subject | Female | - |
dc.subject | CD4 Antigens | - |
dc.subject | Leukocyte Common Antigens | - |
dc.subject | B7-1 Antigen | - |
dc.subject | CD11b Antigen | - |
dc.subject | CD11c Antigen | - |
dc.subject | B7-2 Antigen | - |
dc.title | Recruitment of dendritic cells and macrophages during T cell-mediated synovial inflammation | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1186/ar2328 | - |
pubs.publication-status | Published | - |
Appears in Collections: | Aurora harvest Molecular and Biomedical Science publications |
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hdl_43543.pdf | Published version | 1.5 MB | Adobe PDF | View/Open |
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