Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/43969
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Type: | Journal article |
Title: | The protein tyrosine phosphatase Pez regulates TGFβ, epithelial–mesenchymal transition, and organ development |
Other Titles: | The protein tyrosine phosphatase Pez regulates TGFbeta, epithelial-mesenchymal transition, and organ development |
Author: | Belle, L. Wadham, C. Crocker, L. Lardelli, M. Khew-Goodall, Y. |
Citation: | The Journal of Cell Biology, 2007; 178(7):1223-1235 |
Publisher: | Rockefeller Univ Press |
Issue Date: | 2007 |
ISSN: | 0021-9525 0021-9525 |
Organisation: | Centre for the Molecular Genetics of Development |
Statement of Responsibility: | Leila Wyatt, Carol Wadham, Lesley A. Crocker, Michael Lardelli, and Yeesim Khew-Goodall |
Abstract: | Epithelial–mesenchymal transition (EMT), crucial during embryogenesis for new tissue and organ formation, is also considered to be a prerequisite to cancer metastasis. We report here that the protein tyrosine phosphatase Pez is expressed transiently in discrete locations in developing brain, heart, pharyngeal arches, and somites in zebrafish embryos. We also find that Pez knock-down results in defects in these organs, indicating a crucial role in organogenesis. Overexpression of Pez in epithelial MDCK cells causes EMT, with a drastic change in cell morphology and function that is accompanied by changes in gene expression typical of EMT. Transfection of Pez induced TGFβ signaling, critical in developmental EMT with a likely role also in oncogenic EMT. In zebrafish, TGFβ3 is co- expressed with Pez in a number of tissues and its expression was lost from these tissues when Pez expression was knocked down. Together, our data suggest Pez plays a crucial role in organogenesis by inducing TGFβ and EMT. |
Keywords: | Cell Nucleus Epithelial Cells Embryo, Nonmammalian Mesoderm Animals Zebrafish Dogs Humans Transforming Growth Factor beta Zebrafish Proteins Transcription Factors Oligonucleotides, Antisense RNA, Messenger Signal Transduction Gene Expression Regulation, Developmental Protein Transport Embryonic Development Organogenesis Phenotype Smad4 Protein Transforming Growth Factor beta1 Transforming Growth Factor beta2 Transforming Growth Factor beta3 Protein Tyrosine Phosphatases Protein Tyrosine Phosphatases, Non-Receptor |
Description: | Published online September 24, 2007 |
Rights: | © 2007 Rockefeller University Press |
DOI: | 10.1083/jcb.200705035 |
Published version: | http://dx.doi.org/10.1083/jcb.200705035 |
Appears in Collections: | Aurora harvest 6 Centre for the Molecular Genetics of Development publications Molecular and Biomedical Science publications |
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hdl_43969.pdf | 3.8 MB | Publisher's PDF | View/Open |
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