Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/45129
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Type: Journal article
Title: Integration of transgenes into sexual polyploidization schemes for potato (Solanum tuberosum L.)
Author: Johnson, A.
Veilleux, R.
Citation: Euphytica: international journal on plant breeding, 2003; 133(1):125-138
Publisher: Kluwer Academic Publ
Issue Date: 2003
ISSN: 0014-2336
Organisation: Australian Centre for Plant Functional Genomics (ACPFG)
Statement of
Responsibility: 
Alexander A.T. Johnson & Richard E. Veilleux
Abstract: Most potato transgenic research has focused on development of resistance to pathogens and modification of potato physiology. Many transgenes, particularly those conferring pathogen resistance, could substantially lower potato production costs in developing countries. However, transgenes have not been reported in sexually propagated 4x-2x potato hybrids commonly grown in developing countries. Two transgenes,the Bacillus thuringiensis cry3Aa endotoxin protein gene and the PVY°coat protein gene, were engineered intodiploid and tetraploid potato using Agrobacterium tumefaciens-mediated transformation. Cry3Aa was produced at high levels in several lines while the PVY° coat protein was not expressed. Diplandroid and tetraploid genotypes were crossed to produce transgenic 4x-2x hybrids. Genetic transformation had no discernable effect on fertility ofthe primary transformants, germination of4x-2x seed derived from the transformants and agronomic performance(tuber set, average tuber weight and total tuber yield) of the 4x-2xhybrids. The transgenic 4x-2xhybrids produced non-viable pollen and could only be crossed as female parents. Results suggest that transgenes, such ascry3Aa, could be expressed in 4x-2x hybrids to lower costs of production with no significant effect on plant phenotype.
Keywords: 4x-2x hybrids
cry3Aa
genetic engineering
PVY cp
true potato seed (TPS)
Description: The original publication can be found at www.springerlink.com
DOI: 10.1023/A:1025644523574
Published version: http://dx.doi.org/10.1023/a:1025644523574
Appears in Collections:Aurora harvest
Australian Centre for Plant Functional Genomics publications

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