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https://hdl.handle.net/2440/47323
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Full metadata record
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dc.contributor.author | Spencer, E. | - |
dc.contributor.author | Horiguchi, K. | - |
dc.contributor.author | Khoyi, M. | - |
dc.contributor.author | Sanders, K. | - |
dc.contributor.author | Ward, S. | - |
dc.date.issued | 2002 | - |
dc.identifier.citation | The Journal of Physiology, 2002; 543(3):871-887 | - |
dc.identifier.issn | 0022-3751 | - |
dc.identifier.issn | 1469-7793 | - |
dc.identifier.uri | http://hdl.handle.net/2440/47323 | - |
dc.description | Copyright © 2002 The Physiological Society | - |
dc.description.abstract | Studies of W/WV mice, which lack intramuscular interstitial cells of Cajal (IC-IM), have suggested that IC-IM act as mediators of enteric motor neurotransmission in the gastrointestinal tract. We have studied Sl/Sld mice, which lack the ability to make membrane-bound stem cell factor, to determine the consequences of inappropriate stem cell factor expression on IC-IM populations and on enteric motor neurotransmission. IC-IM were found within the circular and longitudinal muscles of the gastric fundus of wild-type mice. IC-IM were intimately associated with motor nerve terminals and nerve varicosities formed synaptic structures with these cells. IC-IM were also connected with neighbouring smooth muscle cells via gap junctions. Immunohistochemistry and electron microscopy showed that IC-IM were absent from fundus muscles of Sl/Sld mice, but the density of excitatory and inhibitory nerves was not significantly different than in wild-type muscles. Loss of IC-IM was associated with decreased membrane noise (unitary potentials) and significant reductions in post-junctional excitatory and inhibitory enteric nerve responses. Reductions in neural responses were not due to defects in smooth muscle cells as responses to exogenous ACh and K+-induced depolarization were normal in Sl/Sld mice. Responses to neurally released ACh were revealed in Sl/Sld mice by inhibiting ACh breakdown with the acetylcholinesterase inhibitor neostigmine. Inhibitory nerve stimulation elicited inhibitory junction potentials (IJPs) and relaxations in wild-type mice. IJPs were reduced in amplitude and relaxation responses were absent in Sl/Sld mice. These observations suggest that membrane-bound stem cell factor is essential for development of IC-IM and that the close, synaptic-like relationship between nerve terminals and IC-IM may be the primary site of innervation by enteric motor neurons in gastric muscles. | - |
dc.description.statementofresponsibility | Elizabeth A. H. Beckett, Kazuhide Horiguchi, Mohammad Khoyi, Kenton M. Sanders and Sean M. Ward | - |
dc.language.iso | en | - |
dc.publisher | Blackwell Publishing Ltd | - |
dc.source.uri | http://jp.physoc.org/cgi/reprint/543/3/871 | - |
dc.subject | Muscle, Smooth | - |
dc.subject | Gastric Fundus | - |
dc.subject | Motor Neurons | - |
dc.subject | Enteric Nervous System | - |
dc.subject | Animals | - |
dc.subject | Mice | - |
dc.subject | Mice, Mutant Strains | - |
dc.subject | Nitroprusside | - |
dc.subject | Neostigmine | - |
dc.subject | Acetylcholine | - |
dc.subject | Cholinesterase Inhibitors | - |
dc.subject | Vasodilator Agents | - |
dc.subject | Microscopy, Electron | - |
dc.subject | Artifacts | - |
dc.subject | Electric Stimulation | - |
dc.subject | Synaptic Transmission | - |
dc.subject | Excitatory Postsynaptic Potentials | - |
dc.subject | Muscle Tonus | - |
dc.title | Loss of enteric motor neurotransmission in the gastric fundus of Sl/Sld mice | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1113/jphysiol.2002.021915 | - |
pubs.publication-status | Published | - |
Appears in Collections: | Aurora harvest Molecular and Biomedical Science publications |
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