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https://hdl.handle.net/2440/49574
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Type: | Journal article |
Title: | Enrichment of multiphosphorylated peptides by immobilized metal affinity chromatography using Ga(III)- and Fe(III)-complexes |
Author: | Sykora, C. Hoffmann, R. Hoffmann, P. |
Citation: | Protein and Peptide Letters: international journal for rapid publication of short papers in protein and peptide science, 2007; 14(5):489-496 |
Publisher: | Bentham Science Publ Ltd |
Issue Date: | 2007 |
ISSN: | 0929-8665 1875-5305 |
Abstract: | The detection and identification of O-phosphorylation sites in proteins with mass spectrometry remains a challenge. A common approach to analyse these modifications is to enrich phosphopeptides by immobilized metal affinity chromatography (IMAC) prior to mass spectrometric analysis. In this study two commercially available IMAC kits based on Fe(III)-ions immobilized on magnetic beads and Ga(III)-ions immobilized on a chelate-resin, have been investigated and the binding efficiency of peptide mixtures containing non-phosphorylated, singly, doubly and triply phosphorylated peptides have been tested. |
Keywords: | Humans Gallium Ferric Compounds Trypsin Phosphopeptides Ovalbumin tau Proteins Caseins Reagent Kits, Diagnostic Chromatography, Affinity Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization |
DOI: | 10.2174/092986607780782849 |
Description (link): | http://www.bentham.org/ppl/contabs/ppl14-5.htm#14 |
Published version: | http://dx.doi.org/10.2174/092986607780782849 |
Appears in Collections: | Aurora harvest 5 Molecular and Biomedical Science publications |
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