Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/52165
Citations | ||
Scopus | Web of Science® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | Relaxin signalling in primary cultures of human myometrial cells |
Author: | Heng, K. Ivell, R. Wagaarachchi, P. Anand Ivell, R. |
Citation: | Molecular Human Reproduction, 2008; 14(10):603-611 |
Publisher: | Oxford Univ Press |
Issue Date: | 2008 |
ISSN: | 1360-9947 1460-2407 |
Statement of Responsibility: | Kee Heng, Richard Ivell, Prabhath Wagaarachchi and Ravinder Anand-Ivell |
Abstract: | In myometrium of pigs and rats, though not humans, relaxin appears to mediate an inhibition of spontaneous and oxytocin-induced contractility, presumably acting through a G-protein coupled receptor (RXFP1) to generate cAMP. In humans, circulating relaxin is highest in the first trimester, including the time of implantation, when transitory uterine quiescence could help a blastocyst to implant. We investigated whether relaxin can activate adenylate cyclase in primary human myometrial cells from non-pregnant tissue, and we show that relaxin is able to stimulate the generation of cAMP in a manner, which is dependent upon a tyrosine phosphorylation activity, as in the endometrium. We identified transcripts for the relaxin receptor RXFP1 as full-length variants, though a minor splice variant missing exon 2 was also present in low amounts. These cells also express transcripts encoding RXFP2, the receptor for the closely related hormone, INSL3. Although able to respond to relaxin at high concentrations, this receptor does not appear to function by contributing to the cAMP production in human myometrial cells, nor does INSL3 act as a functional agonist or antagonist of relaxin action. In conclusion, the inability of relaxin to inhibit contractility in human myometrial cells would appear to be due to events downstream of simple cAMP generation. |
Keywords: | relaxin human myometrium INSL3 uterine quiescence menstrual cycle |
Description: | Copyright © The Author 2008. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. |
DOI: | 10.1093/molehr/gan051 |
Published version: | http://dx.doi.org/10.1093/molehr/gan051 |
Appears in Collections: | Aurora harvest 5 Molecular and Biomedical Science publications |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.